Busch André, Herten Monika, Haversath Marcel, Kaiser Christel, Brandau Sven, Jäger Marcus
Department of Orthopedics, Trauma and Reconstructive Surgery, St. Marien Hospital Mülheim an der Ruhr, D-45468 Mülheim/Ruhr, Germany.
Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, D-45147 Essen, Germany.
Int J Mol Sci. 2020 Sep 2;21(17):6393. doi: 10.3390/ijms21176393.
During total joint replacement, high concentrations of mesenchymal stromal cells (MSCs) are released at the implantation site. They can be found in cell-tissue composites (CTC) that are regularly removed by surgical suction. A surgical vacuum suction handle was filled with bone substitute granules, acting as a filter allowing us to harvest CTC. The purpose of this study was to investigate the osteopromotive potential of CTC trapped in the bone substitute filter material during surgical suction. In the course of 10 elective total hip and knee replacement surgeries, -tricalcium-phosphate (TCP) and cancellous allograft (Allo) were enriched with CTC by vacuum suction. Mononuclear cells (MNC) were isolated from the CTC and investigated towards cell proliferation and colony forming unit (CFU) formation. Furthermore, MSC surface markers, trilineage differentiation potential and the presence of defined cytokines were examined. Comparable amounts of MNC and CFUs were detected in both CTCs and characterized as MSC‱ of MNC with 9.8 ± 10.7‱ for the TCP and 12.8 ± 10.2‱ for the Allo ( = 0.550). CTCs in both filter materials contain cytokines for stimulation of cell proliferation and differentiation (EGF, PDGF-AA, angiogenin, osteopontin). CTC trapped in synthetic (TCP) and natural (Allo) bone substitute filters during surgical suction in the course of a joint replacement procedure include relevant numbers of MSCs and cytokines qualified for bone regeneration.
在全关节置换过程中，高浓度的间充质基质细胞（MSCs）在植入部位释放。它们可存在于通过手术抽吸定期清除的细胞 - 组织复合物（CTC）中。一个手术用真空抽吸手柄填充有骨替代颗粒，作为过滤器使我们能够收集CTC。本研究的目的是调查在手术抽吸过程中被困在骨替代过滤材料中的CTC的促骨生成潜力。在10例择期全髋关节和膝关节置换手术过程中，通过真空抽吸使磷酸三钙（TCP）和松质骨同种异体移植物（Allo）富集CTC。从CTC中分离出单核细胞（MNC），并研究其细胞增殖和集落形成单位（CFU）形成情况。此外，还检测了MSC表面标志物、三系分化潜能以及特定细胞因子的存在情况。在两种CTC中均检测到相当数量的MNC和CFU，并将其鉴定为MNC的MSC，TCP组为9.8±10.7‱，Allo组为12.8±10.2‱（P = 0.550）。两种过滤材料中的CTC均含有刺激细胞增殖和分化的细胞因子（表皮生长因子、血小板衍生生长因子 - AA、血管生成素、骨桥蛋白）。在关节置换手术过程中，手术抽吸期间被困在合成（TCP）和天然（Allo）骨替代过滤器中的CTC包含大量有资格参与骨再生的MSC和细胞因子。
