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侧向流动免疫层析法对隐球菌病诊断准确性的评估

Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis.

作者信息

Xie Li-Min, Lin Geng-Ling, Dong Hao-Neng, Liao Ying-Xia, Liu Ye-Ling, Qin Jian-Feng, Guo Xu-Guang

机构信息

Department of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, 511436, China.

Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.

出版信息

BMC Infect Dis. 2020 Sep 4;20(1):650. doi: 10.1186/s12879-020-05368-x.

DOI:10.1186/s12879-020-05368-x
PMID:32887568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7472705/
Abstract

BACKGROUND

Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens.

METHODS

Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.

RESULTS

A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.

CONCLUSIONS

Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

摘要

背景

隐球菌是一种引起隐球菌病的条件致病性真菌，隐球菌病是人类面临的最严重的真菌疾病之一。侧向流免疫层析测定法（LFA）已成功应用于隐球菌抗原的快速检测。

方法

于2019年7月之前系统检索Embase、PubMed、Web of Science和Cochrane图书馆中的研究。根据诊断准确性研究质量评估指南，使用Review Manager 5.0评估研究质量。纳入研究中提取的数据通过Meta-DiSc 1.4进行分析。使用Stata 12.0软件检测发表偏倚。

结果

通过纳入和排除标准共纳入15篇文章，其中有31个四格表。血清中的合并敏感性和特异性分别为0.98和0.98，脑脊液中的合并敏感性和特异性分别为0.99和0.99。

结论

与尿液和其他样本相比，血清和脑脊液中的LFA对隐球菌病的诊断具有较高的特异性和敏感性，是有利的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a2f34ae1f226/12879_2020_5368_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/6f04bd520e52/12879_2020_5368_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/9a28f2883814/12879_2020_5368_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/bf24ec6ee396/12879_2020_5368_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a0e27be5df15/12879_2020_5368_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a2c5a1e0b491/12879_2020_5368_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/fcf58f9908ea/12879_2020_5368_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a2f34ae1f226/12879_2020_5368_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/6f04bd520e52/12879_2020_5368_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/9a28f2883814/12879_2020_5368_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/bf24ec6ee396/12879_2020_5368_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a0e27be5df15/12879_2020_5368_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a2c5a1e0b491/12879_2020_5368_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/fcf58f9908ea/12879_2020_5368_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b2/7472705/a2f34ae1f226/12879_2020_5368_Fig7_HTML.jpg

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