College of Food Science and Technology, Huazhong Agricultural University, 1 Shizishan Road, Wuhan, 430070, People's Republic of China.
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, 430070, Hubei, People's Republic of China.
Bioprocess Biosyst Eng. 2021 Feb;44(2):235-246. doi: 10.1007/s00449-020-02437-y. Epub 2020 Sep 4.
Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and α-glucosidase inhibition property in the two standards were also investigated before and after incubated with LAB. The medium contained standards permitted survival of the five LAB stains. All strains exhibited β-glucosidase activity. Of the five LAB strains tested, just L. plantarum N13 and L. brevis LB01 have the ability to metabolize hesperetin-7-O-rutinoside, only L. plantarum N13, L. acidophilus LA85, and L. brevis LB01 could metabolize naringenin-7-O-rutinoside, moreover, L. acidophilus LA85l was the strain with the highest biotransformation ratio of naringenin-7-O-rutinoside. L. acidophilus LA85 and L. plantarum N13 can degrade naringenin-7-O-rutinoside into naringenin. L. brevis LB01 can degrade hesperetin-7-O-rutinoside into hesperetin, 3-(4'-hydroxyphenyl)-2-propenoic acid, 3-(3'-hydroxy-4'-methoxyphenyl)hydracrylic acid, and 3-(4'-hydroxyphenyl)propionic acid. Incubation of L. acidophilus LA85 in naringenin-7-O-rutinoside solution supposed no apparent influence in the biological activities that tested. L. acidophilus LA85 may potentially contribute to the bioavailability of citrus flavanones, and to be applied as functional cultures to obtain more bioavailable and bioactive metabolites in food products or in the human gastrointestinal tract.
微生物过程正在被开发用于将类黄酮糖苷转化为具有更高生物利用度的各种代谢物。本研究旨在确定五种乳酸菌（LAB）菌株（LRhamnosus LRa05、LCasei LC89、LPlantarum N13、LAcidophilus LA85 和 LBrevis LB01）在两种不同柑橘类黄酮标准品（柚皮苷-7-O-鼠李糖苷和柚皮苷-7-O-鼠李糖苷）中的代谢活性和存活率。还研究了在与 LAB 孵育前后两种标准品的酶活性、代谢物、抗氧化活性和α-葡萄糖苷酶抑制特性。培养基中含有允许五种 LAB 菌株存活的标准品。所有菌株均表现出β-葡萄糖苷酶活性。在所测试的五种 LAB 菌株中，只有 LPlantarum N13 和 LBrevis LB01 具有代谢柚皮苷-7-O-鼠李糖苷的能力，只有 LPlantarum N13、LAcidophilus LA85 和 LBrevis LB01 能够代谢柚皮苷-7-O-鼠李糖苷，此外，LAcidophilus LA85l 是具有最高橙皮素-7-O-鼠李糖苷生物转化比的菌株。LAcidophilus LA85 和 LPlantarum N13 可以将橙皮素-7-O-鼠李糖苷降解为橙皮素。LB01 可以将柚皮苷-7-O-鼠李糖苷降解为柚皮苷、3-（4'-羟基苯基）-2-丙烯酸、3-（3'-羟基-4'-甲氧基苯基）羟基丙烯酸和 3-（4'-羟基苯基）丙酸。在橙皮素-7-O-鼠李糖苷溶液中培养 LAcidophilus LA85 对测试的生物活性没有明显影响。LAcidophilus LA85 可能有助于柑橘类黄酮的生物利用度，并作为功能培养物应用于获得食品或人类胃肠道中更具生物利用度和生物活性的代谢物。
