自噬检测方法 III：观察动态蛋白质运输。
Assays for Autophagy III: Observing Dynamic Protein Trafficking.
机构信息
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Methods Mol Biol. 2021;2196:211-222. doi: 10.1007/978-1-0716-0868-5_16.
PMID:32889723
Abstract
Macroautophagy, by its very nature, is a protein trafficking process. Cargos are transported and processed. Atg proteins come and go. In this chapter, we present three assays to monitor these dynamic events: a non-radioactive pulse-chase labeling assay to monitor the transport of prApe1 and two fluorescent microscopy-based assays to assess the trafficking of Atg8 and Atg9.
摘要
自噬本身就是一个蛋白质运输的过程。货物被运输和加工。Atg 蛋白来来去去。在这一章中，我们提出了三种检测方法来监测这些动态事件：一种非放射性脉冲追踪标记实验来监测 prApe1 的运输，以及两种基于荧光显微镜的检测方法来评估 Atg8 和 Atg9 的运输。
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