School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
University of Chinese Academy of Sciences, Beijing, China.
Methods Mol Biol. 2021;2196:235-244. doi: 10.1007/978-1-0716-0868-5_19.
Live-cell imaging is widely used by researchers to study cellular dynamics and obtain a deep understanding of cell biological processes. Keeping cells in the proper growing environment and immobilizing the cells are essential for the imaging of live yeast cells. Here we describe a protocol for monitoring cytoophidia in Saccharomyces cerevisiae and Schizosaccharomyces pombe using inverted confocal fluorescence microscopy. This protocol includes yeast culture, sample preparation, fluorescence imaging, and data analysis.
活细胞成像被研究人员广泛用于研究细胞动力学，深入了解细胞的生物过程。保持细胞在适当的生长环境中并固定细胞对于活酵母细胞的成像至关重要。在这里，我们描述了使用倒置共焦荧光显微镜监测酿酒酵母和裂殖酵母细胞中细胞丝的方案。该方案包括酵母培养、样品制备、荧光成像和数据分析。
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