School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Yeast. 2021 Apr;38(4):276-289. doi: 10.1002/yea.3542. Epub 2021 Jan 28.
CTP synthase (CTPS) cytoophidia have been found in many species over domains of life in the past 10 years, implying the evolutionary conservation of these structures. However, there are differences in cytoophidia between species. The difference in CTPS cytoophidium properties between budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) inspires this research. We study the effects of culture environment on cytoophidia in S. cerevisiae by switching to the optimal medium for S. pombe. S. cerevisiae CTPS cytoophidium fragmentation and pseudohyphae formation are observed after treatment with S. pombe medium YES instead of S. cerevisiae medium YPD. By modifying the level of each ingredient of the media, we find that hypoosmolality impedes cytoophidium integrity during nitrogen starvation. Our study demonstrates the relationship between cytoophidium integrity and environmental stress, supporting the role of cytoophidia in stress resistance.
过去 10 年来，在生命领域的许多物种中都发现了 CTP 合酶（CTPS）细胞体，这意味着这些结构在进化上是保守的。然而，不同物种之间的细胞体存在差异。芽殖酵母（酿酒酵母）和裂殖酵母（裂殖酵母）之间 CTPS 细胞体特性的差异激发了这项研究。我们通过切换到裂殖酵母的最佳培养基来研究培养环境对酿酒酵母细胞体的影响。在用裂殖酵母培养基 YES 代替酿酒酵母培养基 YPD 处理后，观察到酿酒酵母 CTPS 细胞体片段化和假菌丝形成。通过修改培养基中每种成分的水平，我们发现低渗度会在氮饥饿期间阻碍细胞体的完整性。我们的研究表明细胞体完整性与环境应激之间的关系，支持细胞体在应激抗性中的作用。
