School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Int J Mol Sci. 2024 Sep 19;25(18):10092. doi: 10.3390/ijms251810092.
The de novo synthesis of cytidine 5'-triphosphate (CTP) is catalyzed by the enzyme CTP synthase (CTPS), which is known to form cytoophidia across all three domains of life. In this study, we use the budding yeast and the fission yeast as model organisms to compare cytoophidium assembly under external environmental and intracellular CTPS alterations. We observe that under low and high temperature conditions, cytoophidia in fission yeast gradually disassemble, while cytoophidia in budding yeast remain unaffected. The effect of pH changes on cytoophidia maintenance in the two yeast species is different. When cultured in the yeast-saturated cultured medium, cytoophidia in fission yeast disassemble, while cytoophidia in budding yeast gradually form. Overexpression of CTPS results in the presence and maintenance of cytoophidia in both yeast species from the log phase to the stationary phase. In summary, our results demonstrate differential cytoophidium assembly between and , the two most studied yeast species.
三磷酸胞苷（CTP）的从头合成由 CTP 合酶（CTPS）催化，该酶已知在所有三个生命领域中形成细胞丝状结构。在这项研究中，我们使用 budding 酵母 和 fission 酵母 作为模型生物，比较在外部环境和细胞内 CTPS 改变下细胞丝状结构的组装。我们观察到，在低温和高温条件下，fission 酵母中的细胞丝状结构逐渐解体，而 budding 酵母中的细胞丝状结构不受影响。两种酵母中 pH 值变化对细胞丝状结构维持的影响不同。当在酵母饱和培养物中培养时，fission 酵母中的细胞丝状结构解体，而 budding 酵母中的细胞丝状结构逐渐形成。CTPS 的过表达导致细胞丝状结构在两种酵母从对数期到静止期的存在和维持。总之，我们的结果表明，这两种研究最多的酵母物种 和 之间存在细胞丝状结构组装的差异。
