Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil; Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Toxicon. 2020 Nov;187:65-74. doi: 10.1016/j.toxicon.2020.08.021. Epub 2020 Sep 2.
Microgramma vacciniifolia is broadly used in folk medicine but safety information is unavailable. Therefore, we evaluated the toxicity of a saline extract and a lectin-rich fraction of M. vacciniifolia rhizome. The extract showed hemolytic activity on mice erythrocytes at 1000 μg/mL, whereas the fraction promoted hemolysis (8.57-26.15%) at all tested concentrations (10-1000 μg/mL). Acute toxicity test in mice indicated an LD of >5000 mg/kg. Hematological alterations and increased serum alkaline phosphatase level were observed in the treated animals. Transaminases and urea levels increased in the groups treated with the extract or fraction at 5000 mg/kg. Leukocyte infiltration was observed in the liver of extract-treated animals and in the liver and lungs of mice treated with the fraction. The kidneys of animals treated with the fraction at 5000 mg/kg presented hydropic degeneration. The extract and fraction did not induce oxidative stress in the liver and did not show genotoxicity, as examined by micronucleus and comet assays. In conclusion, the preparations were not lethal to mice but caused some signs of toxicity, mainly the fraction. The results indicated the need to evaluate the toxicity of M. vacciniifolia rhizome in other models and in chronic assays.
微果叶千里光在民间医学中被广泛应用，但安全性信息却未知。因此，我们评估了千里光根茎的盐水提取物和富含凝集素的部分的毒性。该提取物在 1000μg/mL 时对小鼠红细胞具有溶血活性，而该部分在所有测试浓度（10-1000μg/mL）下均促进溶血（8.57-26.15%）。急性毒性试验表明，小鼠的 LD>5000mg/kg。在接受治疗的动物中观察到血液学改变和血清碱性磷酸酶水平升高。在接受 5000mg/kg 提取物或部分治疗的组中，转氨酶和尿素水平升高。在接受提取物治疗的动物的肝脏以及接受部分治疗的动物的肝脏和肺部中观察到白细胞浸润。在接受 5000mg/kg 部分治疗的动物的肾脏中观察到水肿变性。提取物和部分在肝脏中均未引起氧化应激，也未通过微核和彗星试验显示出遗传毒性。总之，这些制剂对小鼠没有致死性，但引起了一些毒性迹象，主要是部分。结果表明需要在其他模型和慢性试验中评估千里光根茎的毒性。
