Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Chem Biodivers. 2021 Jun;18(6):e2100125. doi: 10.1002/cbdv.202100125. Epub 2021 May 11.
Previous studies have characterized a saline extract from Microgramma vacciniifolia rhizome and its lectin (MvRL)-rich fraction with low acute toxicity. In the present study, we evaluated these preparations for acute toxicity (1,000 mg/kg) and antinociceptive and anti-inflammatory activities (100-400 mg/kg for the extract and 25-50 mg/kg for the fraction). No signs of toxicity were observed. Both the extract and fraction increased the latency period for nociception in the hot plate assay, decreased writhing induced by acetic acid, and promoted analgesic effects in phases 1 and 2 of the formalin test. The antinociceptive mechanism was attributed to interactions with opioid receptors and K ATPase channels. The extract and fraction decreased carrageenan-induced paw edema in 46.15 % and 77.22 %, respectively, at the highest doses evaluated. Furthermore, the fraction was shown to act on the bradykinin pathway. The ability to decrease leukocyte migration after treatment was also verified in the peritonitis and air pouch models. In exudates collected from air pouches, decreased tumor necrosis factor (TNF)-α and increased interleukin (IL)-10 levels were noted. Both the extract and fraction also effectively inhibited the development of granulomatous tissue. In conclusion, the substances investigated in this study can be used for the development of novel therapeutic options for pain and inflammatory processes.
先前的研究已经对小蔓长春花根茎的盐水提取物及其富含凝集素（MvRL）的部分进行了特征描述，结果表明其具有较低的急性毒性。在本研究中，我们评估了这些制剂的急性毒性（1000mg/kg）和镇痛及抗炎活性（提取物为 100-400mg/kg，部分为 25-50mg/kg）。未观察到毒性迹象。提取物和部分均能延长热板试验中疼痛潜伏期，减少乙酸引起的扭体反应，并促进福尔马林试验的 1 期和 2 期的镇痛作用。镇痛机制归因于与阿片受体和 KATP 通道的相互作用。提取物和部分在最高评估剂量下分别使角叉菜胶诱导的足肿胀减少了 46.15%和 77.22%。此外，部分对缓激肽途径也有作用。在腹膜炎和气囊模型中也验证了治疗后白细胞迁移减少的能力。在气囊渗出物中，观察到肿瘤坏死因子（TNF）-α水平降低，白细胞介素（IL）-10 水平升高。提取物和部分均能有效抑制肉芽组织的形成。综上所述，本研究中研究的物质可用于开发治疗疼痛和炎症过程的新疗法。
