比较短鳍灰鲭鲨（Isurus oxyrinchus）眼和肝的差异表达基因，揭示其单眼视觉和抗癌能力。

Comparative eye and liver differentially expressed genes reveal monochromatic vision and cancer resistance in the shortfin mako shark (Isurus oxyrinchus).

机构信息

Departamento de Ciências do Mar, Instituto do Mar, Universidade Federal de São Paulo - UNIFESP, Rua Carvalho de Mendonça, 144, Santos, São Paulo 11070-100, Brazil; CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, s/n, 4450-208 Porto, Portugal; Departmento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.

Centro de Aquicultura da UNESP, Universidade Estadual Paulista - UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, Jaboticabal, São Paulo 14884-900, Brazil.

出版信息

Genomics. 2020 Nov;112(6):4817-4826. doi: 10.1016/j.ygeno.2020.08.037. Epub 2020 Sep 2.

DOI:10.1016/j.ygeno.2020.08.037

PMID:32890699

Abstract

The shortfin mako, Isurus oxyrinchus is an oceanic pelagic shark found worldwide in tropical and subtropical waters. However, the understanding of its biology at molecular level is still incipient. We sequenced the messenger RNA isolated from eye and liver tissues. De novo transcriptome yielded a total of 705,940 transcripts. A total of 3774 genes were differentially expressed (DEGs), with 1612 in the eye and 2162 in the liver. Most DEGs in the eye were related to structural and signaling functions, including nonocular and ocular opsin genes, whereas nine out of ten most overexpressed genes in the liver were related to tumor suppression, wound healing, and human diseases. Furthermore, DEGs findings provide insights on the monochromatic shark vision and a repertory of cancer-related genes, which may be insightful to elucidate shark resistance to cancer. Therefore, our results provide valuable sequence resources for future functional and population studies.

摘要

短鳍灰鲭鲨，Isurus oxyrinchus，是一种广泛分布于世界各热带和亚热带海域的远洋性上层鲨鱼。然而，其分子水平的生物学理解仍处于初始阶段。我们从眼睛和肝脏组织中分离出的信使 RNA 进行了测序。从头转录组共产生了 705940 个转录本。共有 3774 个基因差异表达（DEGs），其中 1612 个在眼睛中，2162 个在肝脏中。眼睛中的大多数 DEGs 与结构和信号功能有关，包括非眼和眼视蛋白基因，而肝脏中十个最过度表达的基因中有九个与肿瘤抑制、伤口愈合和人类疾病有关。此外，DEGs 的发现为鲨鱼的单色视觉和癌症相关基因库提供了深入的了解，这可能有助于阐明鲨鱼对癌症的抵抗力。因此，我们的研究结果为未来的功能和群体研究提供了有价值的序列资源。