Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China.
Laboratory of Immunopharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China.
J Ethnopharmacol. 2021 Jan 30;265:113345. doi: 10.1016/j.jep.2020.113345. Epub 2020 Sep 3.
Periploca sepium Bunge (P. sepium) is used in traditional Chinese medicine (TCM) for the treatment of autoimmune diseases, particularly rheumatoid arthritis. Periploca sepium periplosides (PePs), isolated from the root bark of P. sepium, characterized as the cardiac glycosides-free pregnane glycosides fraction, is expected to possess therapeutic potential on inflammatory arthritis.
The current study is designed to evaluate the anti-nociceptive, anti-inflammatory and anti-arthritic activities effects of the PePs.
The anti-nociceptive activity of PePs was examined in the writhing test and hot-plate test in mice. The anti-inflammatory activity of PePs was determined by the 2, 4-dinitro-1-fluorobenzene (DNFB)-induced ear edema model and the carrageenan induced paw edema model in mice. The anti-arthritic activity of PePs was investigated by evaluating the joint inflammation and arthritis pathology in rat adjuvant induced arthritis (AIA) and murine collagen induced arthritis (CIA). Phytohaemagglutinin M (PHA-M) -elicited human peripheral blood mononuclear cells (PBMCs) were further applied to assess the suppressive activity of PePs on IFN-γ and IL-17 production.
PePs treatment markedly decreased the acetic acid-induced visceral nociceptive response and increased the hot-plate pain threshold. Further, oral administration of PePs exhibited anti-inflammatory activity by decreasing DNFB-induced ear edema in mice and carrageenan-induced paw edema in rats. Moreover, oral treatment of PePs ameliorated joint swelling and attenuated bone erosion in rodent arthritis, and the therapeutic benefits were partially attributed to the suppression of proinflammatory cytokines such IFN-γ and IL-17. Moreover, PePs suppressed the proliferation as well as IFN-γ and IL-17 secretion in PHA-M-elicited human PBMCs in a concentration dependent manner.
Taken together, our results justified the traditional use of Periploca sepium Bunge for the treatment of diseases associated with inflammation and pain.
杠柳（Periploca sepium Bunge）被用于传统中药（TCM）治疗自身免疫性疾病，特别是类风湿关节炎。杠柳苷（PePs）从杠柳的根皮中分离出来，被认为是无强心苷的孕烷糖苷部分，有望对炎症性关节炎具有治疗潜力。
本研究旨在评估 PePs 的镇痛、抗炎和抗关节炎作用。
在小鼠扭体试验和热板试验中检测 PePs 的镇痛活性。通过 2,4-二硝基-1-氟苯（DNFB）诱导的耳肿胀模型和角叉菜胶诱导的爪肿胀模型测定 PePs 的抗炎活性。通过评估大鼠佐剂性关节炎（AIA）和小鼠胶原诱导关节炎（CIA）中的关节炎症和关节炎病理学，研究 PePs 的抗关节炎活性。进一步应用植物血球凝集素 M（PHA-M）诱导的人外周血单核细胞（PBMC）评估 PePs 对 IFN-γ和 IL-17 产生的抑制活性。
PePs 治疗显著降低了醋酸引起的内脏疼痛反应，提高了热板疼痛阈值。此外，PePs 的口服给药表现出抗炎活性，可降低小鼠的 DNFB 诱导的耳肿胀和大鼠的角叉菜胶诱导的爪肿胀。此外，PePs 治疗改善了啮齿动物关节炎的关节肿胀和骨侵蚀，并部分归因于抑制促炎细胞因子如 IFN-γ和 IL-17。此外，PePs 以浓度依赖的方式抑制 PHA-M 诱导的人 PBMC 的增殖以及 IFN-γ和 IL-17 的分泌。
综上所述，我们的结果证明了杠柳（Periploca sepium Bunge）的传统用途，可用于治疗与炎症和疼痛相关的疾病。
