Wu Moxin, Kim Keun Young, Park Won Cheol, Ryu Han-Seung, Choi Suck Chei, Kim Min Seob, Myung Ji Yeon, Choi Hyun Seok, Kim Eui Joong, Lee Moon Young
Department of Medical Laboratory, Affiliated Hospital of Jiujiang University, Jiujiang 332000, China; Department of Physiology, School of Medicine, Wonkwang University, Wonkwang Digestive Disease Research Institute & Institute of Wonkwang Medical Science, Iksan 54538, Republic of Korea.
Department of General Surgery, Wonkwang University, School of Medicine, Wonkwang Digestive Disease Research Institute, Iksan 54538, Republic of Korea.
Transl Oncol. 2020 Dec;13(12):100861. doi: 10.1016/j.tranon.2020.100861. Epub 2020 Sep 3.
Neurotransmitters are reported to be involved in tumor initiation and progression. This study aimed to elucidate the prognostic value of γ-aminobutyric acid type A receptor δ subunit (GABRD) in colon adenocarcinoma (COAD) using the data from The Cancer Genome Atlas (TCGA) database. The GABRD mRNA expression levels in the COAD and normal tissues were compared using the Wilcoxon rank-sum test. The correlation between clinicopathologic characteristics and GABRD expression was analyzed by Wilcoxon rank-sum test or Kruskal-Wallis test and logistic regression. The prognostic value of GABRD mRNA expression in patients with COAD was determined using the Kaplan-Meier curve and Cox regression analysis. Finally, the molecular mechanisms of GABRD in COAD were predicted by gene set enrichment analysis (GSEA). The COAD tissues exhibited higher GABRD mRNA expression levels than the normal tissues. The logistic regression analysis revealed that GABRD mRNA expression was correlated with TNM stage, N stage, M stage, and microsatellite instability (MSI) status. The Kaplan-Meier survival curve and log-rank test revealed that patients with COAD exhibiting high GABRD mRNA expression were associated with poor overall survival (OS). The multivariate analysis indicated that increased GABRD mRNA expression was an independent prognostic factor and was correlated with a poor OS. The GSEA revealed that GABRD was involved in signaling pathways, including cell adhesion molecules, gap junction, melanogenesis, and mTOR signaling pathway, as well as the signaling pathways associated with basal cell carcinoma or bladder cancer development. In summary, enhanced GABRD mRNA expression may be a potential independent prognostic biomarker for COAD.
据报道，神经递质参与肿瘤的发生和发展。本研究旨在利用癌症基因组图谱（TCGA）数据库的数据阐明γ-氨基丁酸A型受体δ亚基（GABRD）在结肠腺癌（COAD）中的预后价值。采用Wilcoxon秩和检验比较COAD组织和正常组织中GABRD mRNA的表达水平。通过Wilcoxon秩和检验或Kruskal-Wallis检验以及逻辑回归分析GABRD表达与临床病理特征之间的相关性。采用Kaplan-Meier曲线和Cox回归分析确定GABRD mRNA表达在COAD患者中的预后价值。最后，通过基因集富集分析（GSEA）预测GABRD在COAD中的分子机制。COAD组织中GABRD mRNA表达水平高于正常组织。逻辑回归分析显示，GABRD mRNA表达与TNM分期、N分期、M分期和微卫星不稳定性（MSI）状态相关。Kaplan-Meier生存曲线和对数秩检验显示，GABRD mRNA表达高的COAD患者总生存期（OS）较差。多因素分析表明，GABRD mRNA表达增加是一个独立的预后因素，且与较差的OS相关。GSEA显示，GABRD参与细胞黏附分子、间隙连接、黑色素生成和mTOR信号通路等信号通路，以及与基底细胞癌或膀胱癌发生相关的信号通路。总之，GABRD mRNA表达增强可能是COAD潜在的独立预后生物标志物。
