高 MICAL-L2 表达及其在结肠腺癌预后中的作用。
High MICAL-L2 expression and its role in the prognosis of colon adenocarcinoma.
机构信息
The First Clinical Medical College, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Department of Physiology, Nanjing Medical University, 101 Longmian Avenue, Jiangning District, Nanjing, 211166, China.
出版信息
BMC Cancer. 2022 May 2;22(1):487. doi: 10.1186/s12885-022-09614-0.
BACKGROUND
MICAL-like protein 2 (MICAL-L2), a member of the molecules interacting with CasL (MICAL) family of proteins, is strongly associated with the malignancy of multiple types of cancer. However, the role of MICAL-L2 in colon adenocarcinoma (COAD) has not been well characterized.
METHODS
In this study, we analyzed the role of MICAL-L2 in COAD using datasets available from public databases. The mRNA and protein expression of MICAL-L2 was investigated using TCGA, UALCAN, and independent immunohistochemical assays. Overall survival (OS) and disease-specific survival (DSS) of COAD patients were assessed based on the MICAL-L2 expression level using the Kaplan-Meier method. Univariate and multivariate analysis was employed to determine whether MICAL-L2 could serve as an independent prognostic indicator of OS. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) were further utilized to explore the possible cellular mechanism underlying the role of MICAL-L2 in COAD. In addition, the correlation between MICAL-L2 expression and immune cell infiltration levels was investigated via single-sample gene set enrichment analysis (ssGSEA).
RESULTS
Data from TCGA, HPA, and UALCAN datasets indicated that MICAL-L2 expression was significantly higher in COAD tissue than in adjacent normal tissues, and this was confirmed by immunohistochemical assays. Kaplan-Meier survival analysis revealed that patients with MICAL-L2 had shorter OS and DSS. Furthermore, multivariate Cox analysis indicated that MICAL-L2 was an independent risk factor for OS in COAD patients. ROC analysis confirmed the diagnostic value of MICAL-L2, and a prognostic nomogram involving age, M stage, and MICAL-L2 expression was constructed for OS. Functional enrichment analyses revealed that transport-related activity was closely associated with the role of MICAL-L2 in COAD. Regarding immune infiltration levels, MICAL-L2 was found to be positively associated with CD56 NK cells.
CONCLUSIONS
Our results suggested that MICAL-L2 is a promising biomarker for determining prognosis and correlated with immune infiltration levels in COAD.
背景
MICAL 样蛋白 2（MICAL-L2）是分子相互作用与 CasL（MICAL）家族蛋白的成员之一，与多种类型癌症的恶性程度密切相关。然而，MICAL-L2 在结肠腺癌（COAD）中的作用尚未得到很好的描述。
方法
本研究通过公共数据库中的数据集来分析 MICAL-L2 在 COAD 中的作用。使用 TCGA、UALCAN 和独立的免疫组织化学检测来研究 MICAL-L2 的 mRNA 和蛋白表达。根据 MICAL-L2 的表达水平，采用 Kaplan-Meier 法评估 COAD 患者的总生存期（OS）和疾病特异性生存期（DSS）。采用单变量和多变量分析来确定 MICAL-L2 是否可以作为 OS 的独立预后指标。进一步进行基因本体论（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA），以探讨 MICAL-L2 在 COAD 中作用的可能细胞机制。此外，通过单样本基因集富集分析（ssGSEA）来研究 MICAL-L2 表达与免疫细胞浸润水平之间的相关性。
结果
来自 TCGA、HPA 和 UALCAN 数据集的数据表明，MICAL-L2 在 COAD 组织中的表达明显高于相邻正常组织，这通过免疫组织化学检测得到了证实。Kaplan-Meier 生存分析显示，MICAL-L2 表达的患者 OS 和 DSS 更短。此外，多变量 Cox 分析表明，MICAL-L2 是 COAD 患者 OS 的独立危险因素。ROC 分析证实了 MICAL-L2 的诊断价值，并构建了一个包含年龄、M 分期和 MICAL-L2 表达的 OS 预后列线图。功能富集分析表明，与运输相关的活性与 MICAL-L2 在 COAD 中的作用密切相关。关于免疫浸润水平，发现 MICAL-L2 与 CD56 NK 细胞呈正相关。
结论
我们的研究结果表明，MICAL-L2 是一种很有前途的生物标志物，可用于确定 COAD 的预后，并与 COAD 的免疫浸润水平相关。
Zotero 插件