噬菌体P22的E蛋白。I. 从野生型和缺陷型颗粒中的鉴定与释放
E proteins of bacteriophage P22. I. Identification and ejection from wild-type and defective particles.
作者信息
Israel V
出版信息
J Virol. 1977 Jul;23(1):91-7. doi: 10.1128/JVI.23.1.91-97.1977.
Abstract
Of the nine proteins found in the virion of phage P22, four are ejected into the cell after adsorption. The four ejected proteins, termed E proteins, are gp16, gp20, gp26, and gp7. This was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of radioactively labeled phage that had been adsorbed to cells and then eluted off the surface with distilled water. Phage particles that lack gp7 (7- particles) or gp20 (20- particles) successfully eject all their E proteins. The 16- particles do not eject gp7. Analysis of phage ghosts showed that they lack gp16, gp20, and gp7, but they have gp26 in close to normal quantities. Our results suggest roles for gp16 and gp26 in DNA and E protein ejection. All four E proteins are possible candidates for roles in helping the phage DNA cross the plasma membrane.
摘要
在噬菌体P22的病毒粒子中发现的九种蛋白质中，有四种在吸附后被注入细胞。这四种被注入的蛋白质，称为E蛋白，分别是gp16、gp20、gp26和gp7。这是通过对吸附到细胞上然后用蒸馏水从表面洗脱的放射性标记噬菌体进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定的。缺乏gp7（7-颗粒）或gp20（20-颗粒）的噬菌体颗粒成功地注入了它们所有的E蛋白。16-颗粒不注入gp7。对噬菌体空壳的分析表明，它们缺乏gp16、gp20和gp7，但它们的gp26含量接近正常。我们的结果表明gp16和gp26在DNA和E蛋白注入中发挥作用。所有四种E蛋白都可能在帮助噬菌体DNA穿过质膜中发挥作用。
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