School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.
Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
Viruses. 2021 Jul 30;13(8):1504. doi: 10.3390/v13081504.
Tailed double-stranded DNA bacteriophages inject some proteins with their dsDNA during infection. Phage P22 injects about 12, 12, and 30 molecules of the proteins encoded by genes , and , respectively. After their ejection from the virion, they assemble into a trans-periplasmic conduit through which the DNA passes to enter the cytoplasm. The location of these proteins in the virion before injection is not well understood, although we recently showed they reside near the portal protein barrel in DNA-filled heads. In this report we show that when these proteins are missing from the virion, a longer than normal DNA molecule is encapsidated by the P22 headful DNA packaging machinery. Thus, the ejection proteins occupy positions within the virion that can be occupied by packaged DNA when they are absent.
尾部双链 DNA 噬菌体在感染过程中会将一些蛋白质与 dsDNA 一起注入。噬菌体 P22 分别注入约 12、12 和 30 个由基因编码的蛋白质分子、和。从病毒粒子中排出后，它们组装成一个跨周质管，DNA 通过该管进入细胞质。尽管我们最近表明它们位于充满 DNA 的头部的门户蛋白桶附近，但在注入之前这些蛋白质在病毒粒子中的位置尚不清楚。在本报告中，我们表明当这些蛋白质从病毒粒子中缺失时，较长的 DNA 分子被 P22 头部全基因组 DNA 包装机制包被。因此，当它们不存在时，排出蛋白占据病毒粒子内可以被包装 DNA 占据的位置。
