Morel G, Chabot J G, Dubois P M
CNRS UA 559, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Acta Endocrinol (Copenh). 1988 Mar;117(3):307-14. doi: 10.1530/acta.0.1170307.
OT is synthetized in the hypothalamus. These neurons project to the posterior lobe of the pituitary and the external zone of the median eminence. In order to localize OT in the male rat anterior pituitary we have used immunocytochemistry on ultra-thin sections in target cell(s) obtained by cryoultramicrotomy. OT-like immunoreactivity was observed in lactotropes only. No immunoreactivity was observed in gonadotropes, somatotropes, corticotropes or thyrotropes. In lactotropes, immunoreactivity was localized at the plasma membrane level, in the cytoplasmic matrix and around the secretory granules, but not in the other organelles, and in the nucleus. No reaction was observed by using either non-immune serum or anti-OT serum incubated with OT. No modification of OT-like immunoreactivity was observed by using anti-OT serum incubated with heterologous peptides. These results 1) provide immunocytological evidence for the presence of OT in the anterior pituitary gland; 2) indicate the presence of this peptide in one particular cell type, and 3) support the hypothesis that OT could have a direct participation in the regulation of the PRL release.
催产素在下丘脑合成。这些神经元投射到垂体后叶和正中隆起的外侧区。为了在雄性大鼠垂体前叶定位催产素，我们对通过冷冻超薄切片术获得的靶细胞的超薄切片进行了免疫细胞化学研究。仅在催乳素细胞中观察到催产素样免疫反应性。在促性腺激素细胞、生长激素细胞、促肾上腺皮质激素细胞或促甲状腺激素细胞中未观察到免疫反应性。在催乳素细胞中，免疫反应性定位于质膜水平、细胞质基质以及分泌颗粒周围，但在其他细胞器和细胞核中未观察到。使用非免疫血清或与催产素孵育的抗催产素血清均未观察到反应。使用与异源肽孵育的抗催产素血清未观察到催产素样免疫反应性的改变。这些结果1）为垂体前叶中存在催产素提供了免疫细胞学证据；2）表明该肽存在于一种特定的细胞类型中；3）支持催产素可能直接参与催乳素释放调节的假说。
