Guangdong Provincial Key Laboratory of Sports and Health Promotion, Scientific Research Center, Guangzhou Sport University, Guangzhou, China.
Department of Sports and Health, Guangzhou Sport University, Guangzhou, China.
Clin Exp Pharmacol Physiol. 2021 May;48(5):697-703. doi: 10.1111/1440-1681.13404. Epub 2020 Oct 12.
This study investigated the function of perivascular adipose tissue (PVAT) on vascular contractility within resistant arteries in high-fat diet induced obese rats after long-term aerobic exercise. Male Sprague-Dawley rats were subjected to normal diet control group (N-CTRL), normal diet exercise group (N-EX), high-fat diet control group (H-CTRL), and high-fat diet exercise group (H-EX) (n = 8 in each group). After intervention, adipose tissues morphology was observed. Vasomotor function of mesenteric arteries with or without PVAT were assessed; mesenteric PVAT isolated from each group were transferred to chambers bath with untreated vessels (without PVAT) to evaluate the independent effect. Isolated PVAT was further pre-treated with inhibitor of cystathionine-γ-lyase (CSE), a key hydrogen sulphide (H S) enzyme. Results showed that the size of lipid droplet around mesenteric arteries from H-EX was significantly reduced (P < .05); uncoupling protein1 (UCP1) in PVAT from H-EX was enhanced. In N-CTRL, N-EX, and H-EX, vessels without PVAT showed higher sensitivity to serotonin (5-HT) than that with intact PVAT. Vascular tension by 5-HT was significantly reduced in H-EX than H-CTRL (P < .05) in vessels with PVAT. Transferred PVAT from H-EX compared with H-CTRL significantly reduced vascular sensitivity to 5-HT (P < .05), and this effect was eliminated through inhibiting CSE. In summary, the anti-contractile effect of PVAT on resistance artery was impaired in obesity but restored by long-term aerobic exercise. The function of PVAT modified by obesity or by exercise has an independent influence on vascular reactivity, and PVAT derived H S may participate in this process.
这项研究旨在探讨肥胖大鼠长期有氧运动后，血管周围脂肪组织（PVAT）对阻力动脉血管收缩性的作用。雄性 Sprague-Dawley 大鼠分为正常饮食对照组（N-CTRL）、正常饮食运动组（N-EX）、高脂饮食对照组（H-CTRL）和高脂饮食运动组（H-EX）（每组 8 只）。干预后，观察脂肪组织形态。评估肠系膜动脉有无 PVAT 的血管舒缩功能；将各组分离的肠系膜 PVAT 转移至无 PVAT 的血管腔室浴槽中，以评估其独立作用。将分离的 PVAT 用胱硫醚-γ-裂解酶（CSE）抑制剂进一步预处理，CSE 是一种关键的氢硫化物（H S）酶。结果表明，H-EX 大鼠肠系膜动脉周围的脂滴大小明显减小（P <.05）；PVAT 中的解偶联蛋白 1（UCP1）增强。在 N-CTRL、N-EX 和 H-EX 中，无 PVAT 的血管对 5-羟色胺（5-HT）的敏感性高于完整 PVAT 的血管。与 H-CTRL 相比，H-EX 中的血管对 5-HT 的张力明显降低（P <.05）。与 H-CTRL 相比，H-EX 中的转移 PVAT 显著降低了血管对 5-HT 的敏感性（P <.05），而通过抑制 CSE 可消除这种作用。总之，肥胖会损害 PVAT 对阻力动脉的抗收缩作用，但长期有氧运动可使其恢复。肥胖或运动对 PVAT 功能的改变对血管反应性有独立影响，PVAT 衍生的 H S 可能参与了这一过程。
