Shi Hong-Zhuan, Yan Xiao-Lu, Guo Qiao-Sheng, Wang Yi, Yang Pei-Fang, Zhao Tian, Liu Li
Institute of Chinese Medicinal Materials, Nanjing Agricultural University Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2020 Aug;45(16):3812-3818. doi: 10.19540/j.cnki.cjcmm.20200506.108.
The current study was conducted to explore the effects of light intensity in cultivating environment on the cleaning away heat property of Viola yedoensis. In the present study, we established the acute inflammation model of ICR mice by injecting carrageenan. We compared the effects of V. yedoensis grown under different light intensities(100%, 80%, 50%, 35% and 5% of full sunlight) on mice body temperature, thermal radiation and the swelling degree of foot tissue before and after modeling observing by thermal infrared imaging technique and weighing method. The changes of energy metabolism related enzymes in liver were detected by enzyme-linked immunosorbent assay(ELISA). In addition, the effects of V. yedoensis grown under different light intensities on human lung cancer cell A549 proliferation were explored with MTT method. The results showed that the body temperature of all groups of mice in V. yedoensis group were significantly lower than that of the blank group, except 5% full sunlight group, and the body temperature declined in positive proportion to light intensity. V. yedoensis group could alleviate foot swelling, reduce SDH activity in liver tissue(especially 100% full sunlight group and 80% full sunlight group were significantly lower than model group), and the degree of alleviating and reducing was positively correlated with light intensity. There was no significant difference in the activity of Na+-K+-ATPase and Ca(2+)-Mg(2+)-ATPase in liver tissue among treatments. The contents of IL-1β, IL-6, TNF-α, PGE_2 in foot tissue of mice in V. yedoensis groups were significantly lower than those in model group. Among them, the lowest levels of IL-1β, IL-6, TNF-α, PGE_2 were found in 80% full sunlight group, and there was no significant difference in TNF-α among different groups. The effects of V. yedoensis aqueous extract on A549 cell line proliferation inhibition rate increased with the light intensities of V. yedoensis cultivating environment. And the effects of V. yedoensis grown under 100% of full sunlight showed significantly higher A549 cell line proliferation inhibition rate compared with other groups(P<0.05). In summary, the light intensity of V. yedoensis cultivating environment is positively correlated with the cleaning away heat property of V. yedoensis, which conforms to the "light-cold and heat property" hypothesis,The V. yedoensis should be planted under full light according.
本研究旨在探讨栽培环境中的光照强度对紫花地丁清热性能的影响。在本研究中，通过注射角叉菜胶建立ICR小鼠急性炎症模型。利用热红外成像技术和称重法，比较不同光照强度（全日照的100%、80%、50%、35%和5%）下生长的紫花地丁对建模前后小鼠体温、热辐射及足组织肿胀程度的影响。采用酶联免疫吸附测定法（ELISA）检测肝脏中能量代谢相关酶的变化。此外，用MTT法探究不同光照强度下生长的紫花地丁对人肺癌细胞A549增殖的影响。结果显示，紫花地丁组各小鼠体温除全日照5%组外均显著低于空白组，且体温下降与光照强度成正比。紫花地丁组可减轻足肿胀，降低肝脏组织中琥珀酸脱氢酶（SDH）活性（尤其是全日照100%组和80%组显著低于模型组），减轻和降低程度与光照强度呈正相关。各处理组肝脏组织中Na⁺-K⁺-ATP酶和Ca²⁺-Mg²⁺-ATP酶活性无显著差异。紫花地丁组小鼠足组织中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、前列腺素E₂（PGE₂）含量均显著低于模型组。其中，全日照80%组IL-1β、IL-6、TNF-α、PGE₂水平最低，不同组间TNF-α无显著差异。紫花地丁水提取物对A549细胞系增殖抑制率随紫花地丁栽培环境光照强度增加而升高。全日照100%下生长的紫花地丁对A549细胞系增殖抑制率显著高于其他组（P＜0.05）。综上所述，紫花地丁栽培环境光照强度与紫花地丁清热性能呈正相关，符合“光-寒热药性”假说，紫花地丁应在全光照下种植。
