Liu Rui, Wei Shuang, Wang Xin-Zhi, Cheng Jian-Ming, Wu Hao
Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics Nanjing 210023, China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2020 Aug;45(16):3883-3889. doi: 10.19540/j.cnki.cjcmm.20200508.201.
Shotgun based proteomics and peptidomics analysis were used to investigate the proteins and peptides in marine traditional Chinese medicine(TCM) Sepiae Endoconcha(cuttlebone). Peptides were extracted from cuttlebone by acidified methanol, and then strong cation exchange(SCX) resin was used to enrich those peptides. Also, proteins from cuttlebone were extracted and digested by trypsin. nano-LC Q Exactive Orbitrap mass spectrometry was used to analyze proteins and peptides from cuttlebone. As a result, a total of 16 proteins and 168 peptides were identified by protein database search, and 328 peptides were identified by De novo sequencing. The identified proteins were hemocyanin, enolase, myosin, actin, calmodulin, etc., and the identified peptides were derived from actin, histone, and tubulin. All these proteins and peptides were important components in cuttlebone, which would provide important theoretical and research basis for marine TCM cuttlebone investigations.
基于鸟枪法的蛋白质组学和肽组学分析被用于研究海洋中药海螵蛸中的蛋白质和肽。通过酸化甲醇从海螵蛸中提取肽，然后使用强阳离子交换（SCX）树脂富集这些肽。此外，从海螵蛸中提取蛋白质并用胰蛋白酶进行消化。采用纳升液相色谱Q Exactive轨道阱质谱对海螵蛸中的蛋白质和肽进行分析。结果，通过蛋白质数据库搜索共鉴定出16种蛋白质和168种肽，通过从头测序鉴定出328种肽。鉴定出的蛋白质有血蓝蛋白、烯醇化酶、肌球蛋白、肌动蛋白、钙调蛋白等，鉴定出的肽来源于肌动蛋白、组蛋白和微管蛋白。所有这些蛋白质和肽都是海螵蛸中的重要成分，这将为海洋中药海螵蛸的研究提供重要的理论和研究基础。
