Department of Pathology, Beijing Friend Hospital, The Second Clinical Medical College of Capital Medical University, Beijing, China.
Military Medical Science Academy, The PLA Institute of Radiation Medicine, Beijing, China.
J Dermatolog Treat. 2022 May;33(3):1401-1405. doi: 10.1080/09546634.2020.1820935. Epub 2020 Sep 17.
The aim of our study was to investigate the therapeutic effect of Mesenchymal stem cell-derived exosomes (MSC-exs) on eczema mice model.
Eczema mice were established by 2, 4-two nitrochlorobenzene. Human umbilical cords cells and exosomes were harvested. In eczema mice model, the effect of MSC-ex on eczema was evaluated by severity score, atopic dermatitis score and histopathological analysis of dermis. MTT tests were performed to assess PBMC proliferation. Treg was identified by flow cytometry. The angiogenesis was analyzed by endothelial cell tube formation assay.
Compared with PBS, the wound closure of animals treated with MSC-exs was faster. After MSC-exs treatment, there were more new epidermis and dermis, and less scar formation of the lesion. There were significant differences in the integral score of skin injury and the number of lymphocyte infiltration in the skin between the treatment group and the PBS group ( < .01). MSC-exs significantly inhibit Peripheral blood mononuclear cell proliferation, promote the transformation of Treg and the formation of endothelial tube.
MSC-ex accelerated wounds healing in mice eczema model by inhibiting inflammatory cell infiltration and promoting vascular formation.
本研究旨在探讨间充质干细胞衍生的外泌体（MSC-exs）对湿疹小鼠模型的治疗效果。
通过 2,4-二硝基氯苯建立湿疹小鼠模型。分离人脐带细胞和外泌体。在湿疹小鼠模型中，通过严重程度评分、特应性皮炎评分和真皮组织病理学分析评估 MSC-ex 对湿疹的作用。通过 MTT 试验评估 PBMC 增殖。通过流式细胞术鉴定 Treg。通过内皮细胞管状形成试验分析血管生成。
与 PBS 相比，用 MSC-exs 处理的动物的伤口闭合更快。经 MSC-exs 治疗后，病变处有更多的新表皮和真皮，瘢痕形成较少。治疗组与 PBS 组之间皮肤损伤的积分评分和皮肤淋巴细胞浸润数量有显著差异（< .01）。MSC-exs 显著抑制外周血单个核细胞增殖，促进 Treg 的转化和内皮管的形成。
MSC-ex 通过抑制炎症细胞浸润和促进血管形成，加速湿疹小鼠模型的伤口愈合。
