School of Pharmacy (Research Centre of Pharmaceutical Engineering Technology), Harbin University of Commerce, Harbin 150076, People's Republic of China.
J Genet. 2020;99.
Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple have been identified and investigated in numerous plant species. However, few studies have characterized from Dimocarpus longan Lour. In this study, nine from were identified from RNA-Seq data and further verified using the NCBI conserved domain search tool and Pfam database. Bioinformatics tools were used to systematically analyse the physicochemical properties, protein structures, multiple sequence alignment, motif compositions, evolutionary relationships, secondary structures, subcellular localization, phosphorylation sites, signal peptides, GO annotations and protein-protein interactions of the . The expression patterns of the nine were evaluated by qRT-PCR in roots and leaves and in response to varying durations of a 38°C heat treatment. , , and were differentially expressed between root and leaf tissues. All nine DlbZIPs responded to heat treatment in both roots and leaves, but their specific expression levels differed. and were highly expressed in roots after heat treatment, whereas and were highly expressed in leaves after heat treatment. These findings lay a foundation for increasing active secondary metabolite content and improving abiotic stress tolerance in D. longan using transgenic technology.
bZIP 转录因子家族成员在植物发育调控、次生代谢物生物合成以及非生物和生物胁迫响应中发挥着关键作用。迄今为止，在众多植物物种中已经鉴定和研究了多个 bZIP。然而，从龙眼 Dimocarpus longan Lour 中鉴定和研究的 bZIP 较少。在本研究中，从 RNA-Seq 数据中鉴定了龙眼的 9 个 bZIP，并进一步使用 NCBI 保守结构域搜索工具和 Pfam 数据库进行了验证。使用生物信息学工具系统分析了这些 bZIP 的理化性质、蛋白质结构、多重序列比对、基序组成、进化关系、二级结构、亚细胞定位、磷酸化位点、信号肽、GO 注释和蛋白质-蛋白质相互作用。通过 qRT-PCR 在根和叶中以及在不同时长的 38°C 热处理下评估了这 9 个 bZIP 的表达模式。、、和在根和叶组织之间存在差异表达。所有 9 个 DlbZIPs 在根和叶中均对热处理有反应，但它们的特定表达水平不同。在热处理后，和在根中高表达，而和在叶中高表达。这些发现为利用转基因技术提高龙眼主动次生代谢物含量和提高非生物胁迫耐受性奠定了基础。
