Key Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Molecules. 2018 Mar 15;23(3):662. doi: 10.3390/molecules23030662.
Ubiquitin-conjugating enzymes (E2s or UBC enzymes) play vital roles in plant development and combat various biotic and abiotic stresses. Longan ( Lour.) is an important fruit tree in the subtropical region of Southeast Asia and Australia; however the characteristics of the gene family in longan remain unknown. In this study, 40 UBC genes (), which were classified into 15 groups, were identified in the longan genome. An RNA-seq based analysis showed that showed distinct expression in nine longan tissues. Genome-wide RNA-seq and qRT-PCR based gene expression analysis revealed that 11 were up- or down-regualted in the cultivar "Sijimi" (SJ), suggesting that these genes may be important for flower induction. Finally, qRT-PCR analysis showed that the mRNA levels of 13 under SA (salicylic acid) treatment, seven under methyl jasmonate (MeJA) treatment, 27 under heat treatment, and 16 under cold treatment were up- or down-regulated, respectively. These results indicated that the may play important roles in responses to abiotic stresses. Taken together, our results provide a comprehensive insight into the organization, phylogeny, and expression patterns of the longan UBC genes, and therefore contribute to the greater understanding of their biological roles in longan.
泛素结合酶（E2s 或 UBC 酶）在植物发育和抵御各种生物和非生物胁迫中发挥着重要作用。龙眼（Lour.）是东南亚和澳大利亚亚热带地区的一种重要果树；然而，龙眼基因家族的特征尚不清楚。在这项研究中，在龙眼基因组中鉴定出了 40 个泛素结合酶基因（），它们被分为 15 组。基于 RNA-seq 的分析表明，在九个龙眼组织中表现出明显的表达差异。基于全基因组 RNA-seq 和 qRT-PCR 的基因表达分析表明，11 个在品种“四纪米”（SJ）中上调或下调，表明这些基因可能对花诱导很重要。最后，qRT-PCR 分析表明，13 个基因在水杨酸（SA）处理下、7 个基因在茉莉酸甲酯（MeJA）处理下、27 个基因在热处理下、16 个基因在冷处理下的 mRNA 水平分别上调或下调。这些结果表明，这些基因可能在应对非生物胁迫中发挥重要作用。总之，我们的研究结果提供了对龙眼 UBC 基因的组织、系统发育和表达模式的全面了解，因此有助于更好地理解它们在龙眼中的生物学功能。
