Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mol Biol Rep. 2020 Sep;47(9):7097-7106. doi: 10.1007/s11033-020-05776-x. Epub 2020 Sep 7.
Owing to the scarce evidence about the multidrug-resistant (MDR) beta-lactamase-producing Shigella isolates in Iran, this study aimed to evaluate the occurrence of extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in Shigella species collected in the southwest of Iran. This study was conducted on Shigella species isolated from stool samples of pediatric patients aged less than 15 years suffering from diarrhea. These isolates were identified by bacteriology tests, serotyping, and polymerase chain reaction (PCR). The antibiotic resistance was determined by disc diffusion. The production of ESBLs and AmpC was investigated by phenotypic confirmatory tests and PCR. In total, 79 Shigella isolates, including 46.8% (n = 37) of S. flexneri and 53.2% (n = 42) of S. sonnei, were isolated, respectively. The most effective antibiotic was imipenem with 93.7% of susceptibility followed by ampicillin (29.1%), and cotrimoxazole (30.4%).The resistance rates of ceftriaxone, ceftazidime, and cefotaxime were 41.8%, 34.2%, and 41.8%, respectively. Also, a total of 57 (72.2%) isolates showed MDR profiles. The phenotypic tests showed that 43.0% (34/79) of isolates can produce ESBLs, and no one was positive for ApmC. The frequency of bla and bla were 30.4% and 32.9%, respectively, while the bla, bla and AmpC genes were not detected. The ESBL-producing isolates had a significant (p-value ˂ 0.05) resistance rate against ceftriaxone, ceftazidime, cefotaxime, cefepime, erythromycin, and amikacin. The significant prevalence of MDR Shigella isolates harboring ESBL genes highlights the need for effective surveillance measures to prevent the more spread of drug resistance among species.
由于伊朗关于产多药耐药（MDR）β-内酰胺酶的志贺菌分离株的证据很少，因此本研究旨在评估伊朗西南部采集的志贺菌属中扩展谱β-内酰胺酶（ESBL）和AmpC β-内酰胺酶的发生情况。本研究对来自 15 岁以下患有腹泻的儿科患者粪便样本中分离的志贺菌属进行了研究。这些分离株通过细菌学检测、血清分型和聚合酶链反应（PCR）进行鉴定。通过纸片扩散法测定抗生素耐药性。通过表型确证试验和 PCR 研究 ESBL 和 AmpC 的产生。共分离出 79 株志贺菌属，其中 46.8%（n=37）为福氏志贺菌，53.2%（n=42）为宋内志贺菌。最有效的抗生素是亚胺培南，敏感性为 93.7%，其次是氨苄西林（29.1%）和复方新诺明（30.4%）。头孢曲松、头孢他啶和头孢噻肟的耐药率分别为 41.8%、34.2%和 41.8%。此外，共有 57 株（72.2%）分离株表现出 MDR 表型。表型试验显示，43.0%（34/79）的分离株可产生 ESBL，无一株为 AmpC 阳性。bla 和 bla 的检出率分别为 30.4%和 32.9%，而 bla、bla 和 AmpC 基因未检出。产 ESBL 分离株对头孢曲松、头孢他啶、头孢噻肟、头孢吡肟、红霉素和阿米卡星的耐药率显著升高（p 值<0.05）。产 ESBL 的多重耐药志贺菌分离株的显著流行率突出表明，需要采取有效的监测措施，以防止这些耐药基因在不同种属之间进一步传播。
