Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2020 Jul;493(1):201-204. doi: 10.1134/S1607672920040109. Epub 2020 Sep 7.
This work is aimed at studying the mechanisms of reciprocal humoral regulation of noradrenaline-producing organs in rats in the perinatal period of development. The activity of noradrenaline synthesis enzymes tyrosine hydroxylase and dopamine-beta-hydroxylase was measured in the brain and adrenal glands 48 and 72 h after the injection of immunotoxin (anti-dopamine-beta-hydroxylase-saporin) into the rat brain ventricles. It was shown that, 48 h after the immunotoxin injection into the brain, the activity of tyrosine hydroxylase in the brain decreased; however, 72 h after the injection it reached the control levels. This fact indicates that noradrenaline synthesis in the survived neurons increases. In the adrenal glands, 72 h after the immunotoxin injection into the brain, the activity of dopamine-beta-hydroxylase increased. This points to a compensatory increase in the rate of noradrenaline synthesis in the adrenal glands when the synthesis of noradrenaline in the brain is inhibited.
本工作旨在研究大鼠发育围生期去甲肾上腺素产生器官相互体液调节的机制。在向大鼠脑室注射免疫毒素（抗多巴胺-β-羟化酶蓖麻蛋白）48 和 72 h 后，测量脑和肾上腺中去甲肾上腺素合成酶酪氨酸羟化酶和多巴胺-β-羟化酶的活性。结果表明，在向脑内注射免疫毒素 48 h 后，脑内酪氨酸羟化酶的活性降低；然而，在注射后 72 h 时，其达到了对照水平。这一事实表明，存活神经元中的去甲肾上腺素合成增加。在肾上腺中，在向脑内注射免疫毒素 72 h 后，多巴胺-β-羟化酶的活性增加。这表明当脑内去甲肾上腺素合成受到抑制时，肾上腺中去甲肾上腺素合成的速率代偿性增加。
