Department of Orthopaedics, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand Medeze Research and Development, Nakhon Pathom, Thailand.
Eur Rev Med Pharmacol Sci. 2020 Aug;24(16):8273-8280. doi: 10.26355/eurrev_202008_22623.
To describe a novel technique for harvesting mesenchymal stem cells (MSCs) from ankle synovium and demonstrate their multipotency for chondrogenesis and osteogenesis in the treatment of a large osteochondral lesion of the talus (OLT).
MSCs were harvested under local anesthesia from a biopsy of the ankle synovium of a patient with OLT and cultured for 4 weeks. In accordance with the International Society for Cellular Therapy (ISCT) criteria, synovial-derived MSCs were analyzed for cell surface markers using flow cytometry and grown in differentiation induction medium to demonstrate multilineage differentiation potentials in vitro. The patient received an injection of synovial-derived MSCs in a collagen matrix with a fibrin sealant to augment surgery for the lesion.
Harvesting of ankle synovium yielded a culture of 30 million spindle-shaped stem cells in 4 weeks. Cell surface marker expression of the MSCs met the ISCT standards. After 21 days, cultured cells could be differentiated into adipocytes, osteocytes and chondrocytes. The patient recovered uneventfully from surgery and showed satisfactory improvements in pain and ankle motion.
Ankle synovium is a potential source of MSCs for the treatment of OLT. We developed a novel harvesting technique that was simple, convenient and had no complications.
描述一种从踝关节滑膜中采集间充质干细胞（MSCs）的新方法，并展示其在治疗距骨大骨软骨病变（OLT）中的软骨和成骨多能性。
在局部麻醉下从 OLT 患者的踝关节滑膜活检中采集 MSCs 并培养 4 周。根据国际细胞治疗学会（ISCT）标准，使用流式细胞术分析滑膜来源的 MSC 的细胞表面标志物，并在分化诱导培养基中培养以证明体外多能分化潜力。患者接受了用纤维蛋白胶固定在胶原基质中的滑膜来源 MSCs 注射，以增强对病变的手术治疗。
踝关节滑膜采集在 4 周内产生了 3000 万个纺锤形干细胞的培养物。MSC 的细胞表面标志物表达符合 ISCT 标准。21 天后，培养的细胞可分化为脂肪细胞、成骨细胞和成软骨细胞。患者手术恢复顺利，疼痛和踝关节活动度均有满意改善。
踝关节滑膜是治疗 OLT 的 MSCs 的潜在来源。我们开发了一种新的采集技术，简单、方便且无并发症。
