Kim Juhyung, Oh Jeein, Kang Chon-Sik, Choi Youn Soo
Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea.
National Institute of Crop Science, Rural Development Administration (RDA), Wanju 55365, Korea.
Immune Netw. 2020 Jul 15;20(4):e35. doi: 10.4110/in.2020.20.e35. eCollection 2020 Aug.
Antigen delivery systems play critical roles in determining the quality and quantity of Ab responses . Induction of protective antibodies by B cells is essential in the development of vaccines against infectious pathogens, whereas production of IgE antibodies is prerequisite for investigation of allergic responses, or type 1 hypersensitivity reactions. Virus-like particles (VLPs) are efficient platforms for expression of proteins of interest in highly repetitive manners, which grants strong Ab responses to target antigens. Here, we report that delivery of hen egg lysozyme (HEL), a model allergen, through VLP could provoke strong HEL specific IgE Ab responses in mice. Moreover, acute allergic responses were robustly induced in the mice sensitized with VLPs that express HEL, when challenged with recombinant HEL protein. Our data show that antigen delivery in the context of VLPs could function as a platform for sensitization of mice and for subsequent examination of allergic reactions to molecules of interest.
抗原递送系统在决定抗体反应的质量和数量方面起着关键作用。B细胞诱导保护性抗体对于开发针对传染性病原体的疫苗至关重要，而IgE抗体的产生是研究过敏反应或1型超敏反应的先决条件。病毒样颗粒（VLP）是以高度重复方式表达感兴趣蛋白质的有效平台，可引发针对靶抗原的强烈抗体反应。在此，我们报告通过VLP递送模型变应原鸡卵溶菌酶（HEL）可在小鼠中引发强烈的HEL特异性IgE抗体反应。此外，在用表达HEL的VLP致敏的小鼠中，当用重组HEL蛋白攻击时，可强烈诱导急性过敏反应。我们的数据表明，在VLP背景下的抗原递送可作为小鼠致敏以及随后检查对感兴趣分子的过敏反应的平台。
