Laboratoire d'Anatomie, Biomécanique et Organogenèse, Faculté de Médecine, Université Libre de Bruxelles, route de Lennik, 808, B1070 Bruxelles, Belgium.
Laboratoire d'Anatomie, Biomécanique et Organogenèse, Faculté de Médecine, Université Libre de Bruxelles, route de Lennik, 808, B1070 Bruxelles, Belgium.
Morphologie. 2020 Dec;104(347):228-236. doi: 10.1016/j.morpho.2020.07.002. Epub 2020 Sep 4.
Primordial Germ Cells (PGCs) differentiate into spermatozoa or oocytes. They appear early during embryonic development before migrating to the gonadal ridges. Because of their long migration, PGCs have been proposed as a valuable model to study long distance cell migration. Some species also present a vascular phase in the migration of the germline and could therefore be compared to metastatic migration. HSP90 is a heat shock protein involved in the stabilization of several client-proteins, including oncoproteins. HSP90 inhibition has been proved to decrease PGCs migration in mouse and zebrafish.
We investigated the effect of geldanamycin on PGCs migration in a species with a vascular phase, the chicken. Geldanamycin was injected in the egg at 48h of incubation, PGC's were detected in blood using of blood smears, and in the embryo by immunohistochemistry using anti-HSP90 antibody.
The effects of the treatment were similar to those observed in mouse and zebrafish. We show the presence of ectopic germs cells in the vasculature and in the dorsal mesentery, and some deformities of the gonads.
Inhibition of HSP90 decreases the migration of PGCs and proposed the migration of PGCs in the chick embryo as an interesting model to study metastatic invasion.
原始生殖细胞（PGCs）分化为精子或卵子。它们在胚胎发育早期出现，然后迁移到性腺嵴。由于它们的长距离迁移，PGCs 被认为是研究长距离细胞迁移的有价值模型。一些物种的生殖细胞迁移过程中也存在血管相，因此可以与转移性迁移进行比较。HSP90 是一种热休克蛋白，参与稳定包括癌蛋白在内的多种客户蛋白。HSP90 的抑制已被证明可减少小鼠和斑马鱼中 PGCs 的迁移。
我们研究了 geldanamycin 在具有血管相的物种——鸡中对 PGCs 迁移的影响。在孵化 48 小时时将 geldanamycin 注射到鸡蛋中，使用血涂片在血液中检测 PGCs，并通过免疫组织化学使用抗 HSP90 抗体在胚胎中检测。
该处理的效果与在小鼠和斑马鱼中观察到的效果相似。我们显示了血管中和背肠系膜中存在异位生殖细胞，以及一些性腺畸形。
HSP90 的抑制减少了 PGCs 的迁移，并提出了鸡胚胎中 PGCs 的迁移作为研究转移性侵袭的有趣模型。
