Li Dongliang, Yang Shujun, Peng Hongjuan
Department of Pathogen Biology, School of Public Health, Southern Medical University. Guangzhou 510515, China.
Department of Ultrasound Diagnosis, 74th Army Group Hospital, Guangzhou 510318, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2020 May 30;40(5):727-732. doi: 10.12122/j.issn.1673-4254.2020.05.19.
To investigate the changes in the exosomes secreted by mouse dendritic cell line DC2.4 after infection with and to analyze the possible regulatory mechanisms underlying such changes.
The exosomes were extracted by ultracentrifugation from DC2.4 cells at 28 h after infection with . The morphology of the exosomes was examined with transmission electron microscopy, and the exosome size and density were determined using a nanoparticle tracker. High-throughput sequencing was carried out to identify the differentially expressed small RNAs in the exosomes derived from the infected cells.
infection resulted in a significantly increased density of exosomes secreted by DC2.4 cells. Small RNA sequencing revealed that infection caused an increase in the number of miRNAs and piRNAs in the exosomes. The significantly up-regulated piRNAs after the infection included piR-mmu-159, piR-mmu-1526, piR-mmu-9082, piR-mmu-17405, and piR-mmu-25576.
infection causes accumulation and enrichment of exosomes secreted by DC2.4 cells with increased miRNAs and piRNAs in the exosomes.
研究小鼠树突状细胞系DC2.4感染[病原体名称未给出]后分泌的外泌体的变化，并分析这些变化潜在的调控机制。
在DC2.4细胞感染[病原体名称未给出]28小时后，通过超速离心提取外泌体。用透射电子显微镜检查外泌体的形态，并用纳米颗粒跟踪仪测定外泌体的大小和密度。进行高通量测序以鉴定来自感染细胞的外泌体中差异表达的小RNA。
[病原体名称未给出]感染导致DC2.4细胞分泌的外泌体密度显著增加。小RNA测序显示，[病原体名称未给出]感染导致外泌体中miRNA和piRNA数量增加。感染后显著上调的piRNA包括piR-mmu-159、piR-mmu-1526、piR-mmu-9082、piR-mmu-17405和piR-mmu-25576。
[病原体名称未给出]感染导致DC2.4细胞分泌的外泌体积累和富集，外泌体中的miRNA和piRNA增加。
