Orme I M
Department of Microbiology, Colorado State University, Fort Collins 80523.
Clin Exp Immunol. 1988 Mar;71(3):388-93.
Mice were immunized with the cell wall of BCG suspended in an oil-in-saline emulsion, and examined against time for the emergence of T cell-mediated acquired immunity. Evidence is presented that shows that levels of acquired resistance expressed in these animals over the first month following inoculation, and which enabled them to substantially resist an intravenous challenge infection with Mycobacterium tuberculosis, were completely nonspecific in nature, in that they were equally well expressed in normal and T cell-deficient mice, and were present at a time when no protective T cell activity could be passively transferred from the inoculated host. Paradoxically, in contrast, weak but statistically significant protective immunity could be detected in the spleens of CW-immunized mice approximately 3 months after inoculation, at a time when the donor animals were devoid of resistance to rechallenge. Finally, evidence is presented that shows that the CW material, if given subcutaneously, is highly immunogenic for the generation of delayed-type hypersensitivity effector T cells; however, these cells do not themselves contribute to protective immunity.
将悬浮于盐包油乳剂中的卡介苗细胞壁对小鼠进行免疫，并随时间检测T细胞介导的获得性免疫的出现情况。有证据表明，在接种后的第一个月内，这些动物所表现出的获得性抵抗力水平能够使它们基本抵抗结核分枝杆菌的静脉内攻击感染，但其本质上完全是非特异性的，因为在正常小鼠和T细胞缺陷小鼠中表达水平相同，且在无法从接种宿主被动转移保护性T细胞活性时就已存在。相反，矛盾的是，在接种后约3个月时，在经细胞壁免疫的小鼠脾脏中可检测到微弱但具有统计学意义的保护性免疫，此时供体动物对再次攻击没有抵抗力。最后，有证据表明，如果皮下给予细胞壁物质，对于迟发型超敏反应效应T细胞的产生具有高度免疫原性；然而，这些细胞本身并不对保护性免疫起作用。
