Department of Urinary Surgery, Qujing No. 1 People's Hospital, Qujing, Yunnan 655000, China.
Orthopedics Department of Joint Trauma, Yidu Central Hospital of Weifang, Weifang, Shandong 261000, China.
Life Sci. 2020 Dec 1;262:118386. doi: 10.1016/j.lfs.2020.118386. Epub 2020 Sep 6.
The purpose of this paper is to unearth the ceRNA regulatory mechanism of SNHG7 in bladder cancer (BCa).
The expression of SNHG7 in BCa cells was uncovered by qRT-PCR. The biological functions of SNHG7 in BCa cells were explored by CCK-8 assay, colony formation assay, flow cytometry analysis, wound healing assay and transwell assay. Luciferase reporter assay and RIP assay were applied to analyze the interaction of ELK1 with SNHG7 or miR-2682-5p.
SNHG7 was conspicuously highly expressed in BCa tissues and cells. The upregulated expression of SNHG7 was related with poor prognosis in BCa patients. Moreover, SNHG7 exerted oncogenic functions in BCa through enhancing cell growth, migration and invasion. ELK1 increased the level of SNHG7 by binding with the promoter region of SNHG7. SNHG7 strengthened the expression of ELK1 via acting as a sponge of miR-2682-5p. Both ELK1 and miR-2682-5p involved in the SNHG7-mediated BCa progression.
ELK1/SNHG7/miR-2682-5p feedback loop enhances cell growth, migration and invasion in BCa.
本文旨在揭示 SNHG7 在膀胱癌（BCa）中的 ceRNA 调控机制。
通过 qRT-PCR 揭示 SNHG7 在 BCa 细胞中的表达。通过 CCK-8 assay、集落形成 assay、流式细胞术分析、划痕愈合 assay 和 Transwell assay 探究 SNHG7 在 BCa 细胞中的生物学功能。应用荧光素酶报告基因 assay 和 RIP assay 分析 ELK1 与 SNHG7 或 miR-2682-5p 的相互作用。
SNHG7 在 BCa 组织和细胞中显著高表达。SNHG7 的上调表达与 BCa 患者的不良预后相关。此外，SNHG7 通过增强细胞生长、迁移和侵袭在 BCa 中发挥致癌作用。ELK1 通过与 SNHG7 启动子区域结合增加 SNHG7 的水平。SNHG7 通过作为 miR-2682-5p 的海绵增强 ELK1 的表达。ELK1 和 miR-2682-5p 均参与 SNHG7 介导的 BCa 进展。
ELK1/SNHG7/miR-2682-5p 反馈环增强了 BCa 中的细胞生长、迁移和侵袭。
