Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT), Clayton, Panama City 0843-01103, Panama.
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, India.
Mar Drugs. 2020 Sep 3;18(9):456. doi: 10.3390/md18090456.
The marine bacterial genus is known for their ability to produce antimicrobial compounds. The metabolite-producing capacity of has been associated with strain pigmentation; however, the genomic basis of their antimicrobial capacity remains to be explained. In this study, we sequenced the whole genome of six strains (three pigmented and three non-pigmented), with the purpose of identifying biosynthetic gene clusters (BGCs) associated to compounds we detected via microbial interactions along through MS-based molecular networking. The genomes were assembled and annotated using the SPAdes and RAST pipelines and mined for the identification of gene clusters involved in secondary metabolism using the antiSMASH database. Nineteen BGCs were detected for each non-pigmented strain, while more than thirty BGCs were found for two of the pigmented strains. Among these, the groups of genes of nonribosomal peptide synthetases (NRPS) that code for bromoalterochromides stand out the most. Our results show that all strains possess BGCs for the production of secondary metabolites, and a considerable number of distinct polyketide synthases (PKS) and NRPS clusters are present in pigmented strains. Furthermore, the molecular networking analyses revealed two new molecules produced during microbial interactions: the dibromoalterochromides D/D' ().
海洋细菌属以其产生抗菌化合物的能力而闻名。 的代谢产物产生能力与菌株的色素沉着有关；然而，其抗菌能力的基因组基础仍有待解释。在这项研究中，我们对六个 菌株（三个有色素沉着和三个无色素沉着）进行了全基因组测序，目的是识别通过微生物相互作用以及通过基于 MS 的分子网络检测到的化合物相关的生物合成基因簇（BGC）。使用 SPAdes 和 RAST 管道对基因组进行组装和注释，并使用 antiSMASH 数据库挖掘涉及次级代谢的基因簇的鉴定。每个非色素沉着菌株检测到 19 个 BGC，而两个色素沉着菌株则发现了 30 多个 BGC。在这些 BGC 中，编码溴代交替色素的非核糖体肽合酶（NRPS）基因簇最为突出。我们的结果表明，所有菌株都具有产生次生代谢产物的 BGC，并且在色素沉着菌株中存在相当数量的不同聚酮合酶（PKS）和 NRPS 簇。此外，分子网络分析揭示了在微生物相互作用过程中产生的两种新分子：二溴交替色素 D/D'()。
