Wang Mengmeng, Miao Jiaxi, Wang Xuanqing, Li Tuo, Zhu Han, Liu Dongyang, Shen Qirong
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing 210095, China.
Microorganisms. 2020 Sep 4;8(9):1357. doi: 10.3390/microorganisms8091357.
A lignocellulose-degrading strain isolated from thermophilic compost was identified as B5, and found able to secrete considerable amounts of enzymes at optimal temperature (60 °C) and pH (7.5). One circular contig of 3.37 Mbp was assembled from raw data, and 3371 protein-coding genes were predicted. Clusters of orthologous groups (COG) analysis revealed various genes with functions in polymeric substrate degradation, especially for Carbohydrate Active enZymes (CAZymes), such as glycoside hydrolases (GHs) and glycosyl transferases (GTs). Furthermore, the transcriptional responses of B5 at different temperatures-with rice straw provided as the sole carbon source-were analyzed. The results revealed that B5 could resist high temperature by upregulating heat shock proteins (HSPs), enhancing protein synthesis, and decreasing carbon catabolism. Briefly, B5 possesses the ability of lignocellulose degradation, and might be considered a potential inoculant for improving composting efficiency.
从嗜热堆肥中分离出的一种木质纤维素降解菌株被鉴定为B5，发现其在最佳温度（60°C）和pH值（7.5）下能够分泌大量酶。从原始数据中组装出一个3.37 Mbp的环状重叠群，并预测了3371个蛋白质编码基因。直系同源群（COG）分析揭示了各种在聚合物底物降解中起作用的基因，特别是对于碳水化合物活性酶（CAZyme），如糖苷水解酶（GH）和糖基转移酶（GT）。此外，分析了B5在以稻草为唯一碳源的不同温度下的转录反应。结果表明，B5可以通过上调热休克蛋白（HSP）、增强蛋白质合成和减少碳分解代谢来抵抗高温。简而言之，B5具有木质纤维素降解能力，可能被视为提高堆肥效率的潜在接种剂。
