钙磷和溶磷菌对香榧枝废弃物堆肥过程中微生物结构和功能的影响。
Effects of calcium phosphate and phosphorus-dissolving bacteria on microbial structure and function during Torreya Grandis branch waste composting.
机构信息
State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou, China.
School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China.
出版信息
BMC Microbiol. 2024 Oct 2;24(1):385. doi: 10.1186/s12866-024-03535-7.
UNLABELLED
BACKGROUND BURKHOLDERIA: is a phosphorus solubilizing microorganism discovered in recent years, which can dissolve insoluble phosphorus compounds into soluble phosphorus. To investigate the effects of Burkholderia and calcium phosphate on the composting of Torreya grandis branches and leaves, as well as to explain the nutritional and metabolic markers related to the composting process.
METHODS
In this study, we employed amplicon sequencing and untargeted metabolomics analysis to examine the interplay among phosphorus (P) components, microbial communities, and metabolites during T. grandis branch and leaf waste composting that underwent treatment with calcium phosphate and phosphate-solubilizing bacteria (Burkholderia). There were four composting treatments, 10% calcium phosphate (CaP) or 5 ml/kg (1 × 10/ml Burkholderia) microbial inoculum (WJP) or both (CaP + WJP), and the control group (CK).
RESULTS
The results indicated that Burkholderia inoculation and calcium phosphate treatment affected the phosphorus composition, pH, EC, and nitrogen content. Furthermore, these treatments significantly affected the diversity and structure of bacterial and fungal communities, altering microbial and metabolite interactions. The differential metabolites associated with lipids and organic acids and derivatives treated with calcium phosphate treatment are twice as high as those treated with Burkholderia in both 21d and 42d. The results suggest that calcium phosphate treatment alters the formation of some biological macromolecules.
CONCLUSION
Both Burkholderia inoculation and calcium phosphate treatment affected the phosphorus composition, nitrogen content and metabolites of T. grandis branch and leaf waste compost.These results extend our comprehension of the coupling of matter transformation and community succession in composting with the addition of calcium phosphate and phosphate-solubilizing bacteria.
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背景
伯克霍尔德氏菌是近年来发现的一种能溶解磷的微生物，它能将不溶性磷化合物转化为可溶性磷。为了研究伯克霍尔德氏菌和磷酸钙对香榧树枝叶堆肥的影响，并解释与堆肥过程相关的营养和代谢标志物。
方法
本研究采用扩增子测序和非靶向代谢组学分析，研究了在添加磷酸钙和溶磷菌（伯克霍尔德氏菌）的情况下，香榧树枝叶废物堆肥过程中磷成分、微生物群落和代谢物之间的相互作用。有四种堆肥处理，分别为 10%磷酸钙（CaP）或 5ml/kg（1×10/ml 伯克霍尔德氏菌）微生物接种物（WJP）或两者（CaP+WJP），以及对照组（CK）。
结果
结果表明，伯克霍尔德氏菌接种和磷酸钙处理影响了磷组成、pH 值、电导率和氮含量。此外，这些处理还显著影响了细菌和真菌群落的多样性和结构，改变了微生物和代谢物的相互作用。在 21d 和 42d 时，用磷酸钙处理的差异代谢物与脂质和有机酸及其衍生物的相关性是用伯克霍尔德氏菌处理的两倍。结果表明，磷酸钙处理改变了一些生物大分子的形成。
结论
伯克霍尔德氏菌接种和磷酸钙处理都影响了香榧树枝叶废物堆肥的磷组成、氮含量和代谢物。这些结果扩展了我们对添加磷酸钙和溶磷菌后堆肥中物质转化和群落演替耦合的理解。
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