严重急性呼吸综合征冠状病毒2（新冠病毒）检测：综述

Testing for SARS-CoV-2 (COVID-19): A General Review.

作者信息

Tang Eric W, Bobenchik April M, Lu Shaolei

机构信息

Department of Pathology and Laboratory Medicine, Alpert Medical School of Brown University, Providence, RI.

出版信息

R I Med J (2013). 2020 Sep 4;103(8):20-23.

PMID:32900007

Abstract

The rampant COVID-19 pandemic has strained the testing capabilities of healthcare centers across the country. Several nucleic acid and serologic assays are available or currently being developed to meet the growing demand for large-scale testing. This review summarizes the developments of commonly used testing methods and their strategic use in clinical diagnosis and epidemiologic surveillance. This review will cover the basic virology of SARS-CoV-2, nucleic acid amplification testing, serology, antigen testing, as well as newer testing methods such as CRISPR-based assays.

摘要

肆虐的新冠疫情使全国医疗中心的检测能力不堪重负。为满足大规模检测不断增长的需求，已有多种核酸和血清学检测方法可供使用或正在研发。本综述总结了常用检测方法的进展及其在临床诊断和流行病学监测中的策略性应用。本综述将涵盖严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的基础病毒学、核酸扩增检测、血清学、抗原检测以及基于成簇规律间隔短回文重复序列（CRISPR）的检测等较新的检测方法。