Mendes N F
Lymphology. 1977 Jun;10(2):85-93.
Lymphocytes can be subdivided into two major groups according to origin and function (1,2). T lymphocytes are thymus dependent and are responsible for cell-mediated immunity while the B lymphocytes represent precursors of immunoglobulin-producing plasma cells and give rise to humoral immunity. Considerable knowledge concerning the biological properties of T and B lymphocytes and their mutual interaction has been obtained in the last few years. Recent data also demonstrate further heterogeneity within each of the two main lymphocyte populations. Certain lymphoid cells are capable of mediating cytotoxicity against target cells coated with specific antibody. These cells are termed K cells, and although some evidence seems to suggest that they comprise a subpopulation of B lymphocytes, their nature and localization in lymphoid organ is not well known. This article will attempt to cover the methodologies involved in assays of T, B, and K cells. The methods to be discussed are used to detect the presence of different lymphoid cell populations; the markers used are not necessarily related to cell function.
淋巴细胞可根据起源和功能分为两大类(1,2)。T淋巴细胞依赖胸腺，负责细胞介导的免疫，而B淋巴细胞是产生免疫球蛋白的浆细胞的前体，产生体液免疫。在过去几年中，已经获得了关于T和B淋巴细胞的生物学特性及其相互作用的大量知识。最近的数据还表明，在这两个主要淋巴细胞群体中，各自都存在进一步的异质性。某些淋巴细胞能够对包被有特异性抗体的靶细胞介导细胞毒性。这些细胞被称为K细胞，尽管一些证据似乎表明它们构成了B淋巴细胞的一个亚群，但它们在淋巴器官中的性质和定位尚不清楚。本文将试图涵盖T、B和K细胞检测方法。所讨论的方法用于检测不同淋巴细胞群体的存在；所使用的标志物不一定与细胞功能相关。
