Exercise Physiology Research Group, Department of Movement Sciences, Faculty of Movement and Rehabilitation Sciences, Catholic University of Leuven, Leuven, Belgium.
Physiol Res. 2020 Nov 16;69(5):847-859. doi: 10.33549/physiolres.934482. Epub 2020 Sep 9.
Muscle regeneration is regulated through interaction between muscle and immune cells. Studies showed that treatment with supra-physiological doses of Non-Steroidal Anti-Inflammatory Drug (NSAID) abolished inflammatory signaling and impaired muscle recovery. The present study examines the effects of pharmacologically-relevant NSAID treatment on muscle regeneration. C57BL/6 mice were injected in the tibialis anterior (TA) with either PBS or cardiotoxin (CTX). CTX-injected mice received ibuprofen (CTX-IBU) or were untreated (CTX-PLAC). After 2 days, Il-1beta and Il-6 expression was upregulated in the TA of CTX-IBU and CTX-PL vs. PBS. However, Cox-2 expression and macrophage infiltration were higher in CTX-PL vs. PBS, but not in CTX-IBU. At the same time, anabolic markers were higher in CTX-IBU vs. PBS, but not in CTX-PL. Nevertheless, ibuprofen did not affect muscle mass or muscle fiber regeneration. In conclusion, mild ibuprofen doses did not worsen muscle regeneration. There were even signs of a transient improvement in anabolic signaling and attenuation of inflammatory signaling.
肌肉再生是通过肌肉和免疫细胞之间的相互作用来调节的。研究表明，超生理剂量的非甾体抗炎药（NSAID）治疗会消除炎症信号，并损害肌肉恢复。本研究检查了药理相关的 NSAID 治疗对肌肉再生的影响。C57BL/6 小鼠在前胫骨肌（TA）中注射 PBS 或心脏毒素（CTX）。CTX 注射的小鼠接受布洛芬（CTX-IBU）或未治疗（CTX-PLAC）。2 天后，CTX-IBU 和 CTX-PL 中的 IL-1beta 和 IL-6 表达在上皮 TA 中上调，而不是 PBS。然而，在 CTX-PL 中，cox-2 表达和巨噬细胞浸润高于 PBS，但在 CTX-IBU 中则没有。与此同时，CTX-IBU 中的合成代谢标记物高于 PBS，但在 CTX-PL 中则没有。然而，布洛芬并没有影响肌肉质量或肌纤维再生。总之，轻度布洛芬剂量不会加重肌肉再生。甚至有迹象表明，合成代谢信号的短暂改善和炎症信号的衰减。
