在动物模型中使用含环丙沙星的壳聚糖植入物预防开放性骨折后的骨感染
Prevention of bone infection after open fracture using a chitosan with ciprofloxacin implant in animal model.
作者信息
Kobata Silvia Iovine, Teixeira Luiz Eduardo Moreira, Fernandes Simone Odilia Antunes, Faraco André Augusto Gomes, Vidigal Paula Vieira Teixeira, Araújo Ivana Duval de
机构信息
Fellow Master degree, Postgraduate Program in Sciences Applied to Surgery and Ophthalmology Academic Unit, School of Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil. Acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript preparation and writing.
PhD, Full Professor, Locomotor System Department, Medical School, UFMG, Belo Horizonte-MG, Brazil. Critical revision, final approval.
出版信息
Acta Cir Bras. 2020 Sep 4;35(8):e202000803. doi: 10.1590/s0102-865020200080000003.
PURPOSE
To evaluate different concentrations of ciprofloxacin to prevent infection after open fracture contaminated with S. aureus in rats using absorbable local delivery system.
METHODS
Fifty-two Wistar rats were assigned to six groups. After 4 weeks, all animals underwent 99mTc-ceftizoxima scintigraphy evaluation, callus formation measurement and histological analysis. ANOVA, t-Student and Kruskal Wallis were used for quantitative variables statistical analysis, whereas qui square and exact Fisher were used for qualitative variables.
RESULTS
Treatment using 25% and 50% of ciprofloxacin incorporated at the fracture fixation device were effective in preventing bone infection compared to control group (p<0.05). Chitosan were not effective in preventing bone infection when used alone compared to control group (p>0.05). Histological findings demonstrated bone-healing delay with 50% of ciprofloxacin. No difference in callus formation were observed (p>0.05).
CONCLUSION
Local delivery treatment for contaminated open fracture using chitosan with ciprofloxacin is effective above 25%.
目的
使用可吸收局部给药系统评估不同浓度的环丙沙星对大鼠金黄色葡萄球菌污染开放性骨折后预防感染的效果。
方法
将52只Wistar大鼠分为六组。4周后，所有动物均接受99mTc-头孢唑肟闪烁扫描评估、骨痂形成测量和组织学分析。方差分析、t检验和Kruskal Wallis检验用于定量变量的统计分析，而卡方检验和精确Fisher检验用于定性变量。
结果
与对照组相比，在骨折固定装置中加入25%和50%环丙沙星的治疗对预防骨感染有效（p<0.05）。与对照组相比，单独使用壳聚糖预防骨感染无效（p>0.05）。组织学结果显示，50%环丙沙星导致骨愈合延迟。骨痂形成未见差异（p>0.05）。
结论
壳聚糖与环丙沙星联合用于污染开放性骨折的局部给药治疗，浓度高于25%时有效。
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