慢性酒精中毒与糖尿病共同作用诱导大鼠海绵体发生细胞凋亡。
Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats.
作者信息
Schimming Bruno Cesar, Cirino Mucio Luiz de Assis, Lizarte Neto Fermino Sanches, Novais Paulo Cezar, Carvalho Camila Albuquerque Melo de, Tirapelli Daniela Pretti da Cunha, Molina Carlos Augusto Fernandes, Tirapelli Luis Fernando, Tucci Junior Silvio
机构信息
PhD, Associate Professor, Department of Anatomy, Biosciences Institute of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Technical procedures, interpretation of data, statistical analysis, manuscript preparation, critical revision.
Fellow PhD degree, Postgraduate Program in Surgical Clinic, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, statistical analysis.
出版信息
Acta Cir Bras. 2020 Sep 7;35(8):e202000805. doi: 10.1590/s0102-865020200080000005.
PURPOSE
To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum.
METHODS
Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR.
RESULTS
The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups.
CONCLUSIONS
The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.
目的
评估酒精暴露和糖尿病对阴茎海绵体凋亡过程的影响。
方法
将48只雄性Wistar大鼠分为四组：对照组、糖尿病组、酒精组和糖尿病 - 酒精组。制备阴茎海绵体样本，通过免疫组织化学和实时定量聚合酶链反应研究凋亡基因（半胱天冬酶 - 3和9）的蛋白表达。
结果
半胱天冬酶 - 3和 - 9的免疫反应性呈弥漫性，且在治疗组中更高，尽管实验组之间无显著差异，仅与对照组相比时有差异。与对照组和乙醇组相比，糖尿病组和乙醇 - 糖尿病组中半胱天冬酶 - 9的基因表达增加。
结论
这些因素（乙醇和糖尿病）的联合可能会影响大鼠阴茎海绵体组织病变中的凋亡机制。糖尿病组和乙醇 - 糖尿病组中半胱天冬酶 - 9的基因和蛋白表达均表明该研究模型涉及凋亡级联反应。
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