Dechesne C J, Thomasset M, Brehier A, Sans A
INSERM - U. 254, Laboratoire de Neurophysiologie Sensorielle, USTL, Montpellier, France.
Hear Res. 1988 Jun;33(3):273-8. doi: 10.1016/0378-5955(88)90157-8.
Previous reports on calbindin, a 28 kDa vitamin D-induced calcium-binding protein, located in the mammalian peripheral vestibular system indicated that it is specifically distributed and postulated that it could play a role in the electrophysiological functioning of the sensory cells. This immunocytochemical investigation of the distribution of calbindin in the vestibular system of various vertebrates: fishes (goldfish and sea-perch), amphibia (frog), birds (chicken) and mammals (mouse, cat and baboon), was performed to verify these observations. In the vestibular ganglion, only a few neurons were faintly immunoreactive in the fishes and the frog, while the staining was more intense but still not present in all neurons of the chicken, the mouse and the cat. All the neurons were immunoreactive in the baboon. No immunoreactivity was observed in the sensory epithelia of the fishes. All hair cells were strongly immunoreactive in the frog. In the other species, most of the hair cells in the cristae were immunostained except those situated in the peripheral areas. In the maculae, the hair cells of the striola were either the only ones stained or were more intensely stained or were more intensely stained than the others. The localization of calbindin in specific cellular types and its increasing abundance from the fishes to the mammals suggest that calbindin is associated with the capacity of sensory and nerve cells to analyze precise mechanical or biochemical stimulations.
先前关于钙结合蛋白（一种28 kDa的维生素D诱导的钙结合蛋白）的报道表明，它位于哺乳动物外周前庭系统中，具有特定的分布，并推测它可能在感觉细胞的电生理功能中发挥作用。本研究通过免疫细胞化学方法，对钙结合蛋白在各种脊椎动物（鱼类（金鱼和海鲈）、两栖动物（青蛙）、鸟类（鸡）和哺乳动物（小鼠、猫和狒狒））前庭系统中的分布进行了研究，以验证这些观察结果。在前庭神经节中，鱼类和青蛙只有少数神经元呈微弱免疫反应，鸡、小鼠和猫的染色较强，但并非所有神经元都有染色。狒狒的所有神经元均呈免疫反应。在鱼类的感觉上皮中未观察到免疫反应。青蛙的所有毛细胞均呈强免疫反应。在其他物种中，嵴上的大多数毛细胞都有免疫染色，但位于周边区域的毛细胞除外。在椭圆囊斑中，中央带的毛细胞要么是唯一染色的，要么染色比其他毛细胞更强。钙结合蛋白在特定细胞类型中的定位及其从鱼类到哺乳动物丰度的增加表明，钙结合蛋白与感觉细胞和神经细胞分析精确机械或生化刺激的能力有关。
