Department of Forensic Medicine, Wroclaw Medical University, 4 J. Mikulicza-Radeckiego Street, 50-345 Wrocław, Poland.
Institute of Toxicology Research, 45 Kasztanowa Street, 55-093 Borowa, Poland.
J Anal Toxicol. 2021 Jul 10;45(6):605-611. doi: 10.1093/jat/bkaa123.
This paper presents a rapid, sensitive and precise method developed and validated for the quantification of sufentanil in biological samples using ultra-performance liquid chromatography coupled with QqQ-MS-MS. Plasma samples were extracted with simple and fast liquid-liquid extraction (ethyl acetate, pH 9). Calibration curve showed linearity in the concentration range of 0.005-30 µg/L. The lower limit of quantification was 0.010 µg/L. The most important method features are low lower limit of quantification value, simple plasma extraction and small sample volume. This method is suitable not only for evaluation of the pharmacokinetics, toxicology, bioavailability and clinical pharmacology of sufentanil but also for the detection and identification of this compound in human plasma samples for forensic purposes.
本文提出了一种快速、灵敏、准确的方法，用于使用超高效液相色谱-串联质谱法（UPLC-QqQ-MS-MS）定量生物样品中的舒芬太尼。血浆样品采用简单快速的液-液萃取（乙酸乙酯，pH9）进行提取。校准曲线在 0.005-30µg/L 的浓度范围内呈线性。定量下限为 0.010µg/L。该方法最重要的特点是具有较低的定量下限值、简单的血浆提取和小体积的样本。该方法不仅适用于舒芬太尼的药代动力学、毒理学、生物利用度和临床药理学评价，也适用于法医学中检测和鉴定人血浆样品中的该化合物。
