WHO Collaborating Center for Reference and Research on Bacterial Pathogens, Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Ministry of Public Health, Beirut, Lebanon.
J Infect Dev Ctries. 2020 Aug 31;14(8):878-885. doi: 10.3855/jidc.12072.
Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in Lebanon are scarce. This study was undertaken to reveal the spoligotypes of MTB isolates recovered from patients in Lebanon.
MTB isolates from 49 patients living in Lebanon were recovered and identified. The samples were heat killed and subjected to DNA extraction. Spoligotyping was performed using microbeads from TB-SPOL Kit and the fluorescence intensity was measured using Luminex 200®. Generated patterns were assigned to families using the SITVIT2 international database of the Pasteur Institute of Guadeloupe and compared.
The spoligotyping of the 49 MTB isolates revealed that 31 isolates belonged to Lineage 4 (Euro-American, 63.3%), 12 to Lineage 3 (East- African Indian, 24.5%), 3 to Lineage 2 (East Asian, 6%) and 2 were unknown. Over half of the genotypes (16 of 30) harbored SIT127 supposed to belong to the L4.5 sublineage. One isolate belonging to the rare Manu-Ancestor SIT523 was recovered for the first time in Lebanon, being associated with highly virulent extensively drug-resistant (XDR) MTB phenotype.
The application of the Spoligotyping Multiplex Luminex® method is an efficient, discriminatory and rapid method to use for first-lane genotyping of MTB isolates. Though humble numbers were tested, this study is one of the first to describe the genomic diversity and epidemiology of MTB isolates of Lebanon, and suggests an increasing prevalence of SIT127 in the country.
黎巴嫩有关循环分枝杆菌分离株（MTB）基因型的数据很少。本研究旨在揭示从黎巴嫩患者中回收的 MTB 分离株的 spoligotypes。
从居住在黎巴嫩的 49 名患者中回收并鉴定 MTB 分离株。将样本加热灭活并进行 DNA 提取。使用 TB-SPOL 试剂盒中的微珠进行 spoligotyping，并使用 Luminex 200®测量荧光强度。使用瓜德罗普巴斯德研究所的 SITVIT2 国际数据库分配生成的图案家族，并进行比较。
49 株 MTB 分离株 spoligotyping 显示，31 株属于谱系 4（欧洲-美洲，63.3%），12 株属于谱系 3（东非印度，24.5%），3 株属于谱系 2（东亚，6%），2 株未知。超过一半的基因型（30 个中的 16 个）含有 SIT127，据推测属于 L4.5 亚谱系。首次在黎巴嫩回收了属于罕见 Manu-Ancestor SIT523 的一株分离株，与高度毒力广泛耐药（XDR）MTB 表型相关。
Spoligotyping Multiplex Luminex®方法是一种高效、有区别且快速的方法，可用于 MTB 分离株的一线基因分型。尽管测试的数量很少，但本研究首次描述了黎巴嫩 MTB 分离株的基因组多样性和流行病学，并表明 SIT127 在该国的流行率不断增加。
