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IAA15的一个功能获得性突变体通过转录抑制拟南芥中的基因来抑制侧根发育。

A Gain-of-Function Mutant of IAA15 Inhibits Lateral Root Development by Transcriptional Repression of Genes in Arabidopsis.

作者信息

Kim Sun Ho, Bahk Sunghwa, An Jonguk, Hussain Shah, Nguyen Nhan Thi, Do Huy Loc, Kim Jae-Yean, Hong Jong Chan, Chung Woo Sik

机构信息

Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, South Korea.

出版信息

Front Plant Sci. 2020 Aug 12;11:1239. doi: 10.3389/fpls.2020.01239. eCollection 2020.

DOI:10.3389/fpls.2020.01239
PMID:32903377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7434933/
Abstract

Lateral root development is known to be regulated by Aux/IAA-ARF modules in . As components, several Aux/IAAs have participated in these Aux/IAA-ARF modules. In this study, to identify the biological function of IAA15 in plant developments, transgenic plant overexpressing the gain-of-function mutant of IAA15 (IAA15 OX) under the control of dexamethasone (DEX) inducible promoter, in which IAA15 protein was mutated by changing Pro-75 residue to Ser at the degron motif in conserved domain II, was constructed. As a result, we found that IAA15 OX plants show a decreased number of lateral roots. Coincidently, promoter-GUS reporter analysis revealed that transcripts were highly detected in all stages of developing lateral root tissues. It was also verified that the IAA15 protein is strongly stabilized against proteasome-mediated protein degradation by inhibiting its poly-ubiquitination, resulting in the transcriptional repression of auxin-responsive genes. In particular, transcript levels of and , which are positive regulators of lateral root formation, dramatically repressed in IAA15 OX plants. Furthermore, it was elucidated that IAA15 interacts with ARF7 and ARF19 and binds to the promoters of and , strongly suggesting that IAA15 represses lateral root formation through the transcriptional suppression of and by inhibiting ARF7 and ARF19 activity. Taken together, this study suggests that IAA15 also plays a key negative role in lateral root formation as a component of Aux/IAA-ARF modules.

摘要

已知侧根发育受Aux/IAA-ARF模块调控。作为这些模块的组成部分，几种Aux/IAA蛋白参与其中。在本研究中，为了确定IAA15在植物发育中的生物学功能，构建了在糖皮质激素（DEX）诱导型启动子控制下过表达IAA15功能获得性突变体（IAA15 OX）的转基因植物，其中IAA15蛋白在保守结构域II的降解基序处通过将第75位脯氨酸残基突变为丝氨酸而发生突变。结果，我们发现IAA15 OX植物的侧根数量减少。巧合的是，启动子-GUS报告基因分析显示，在侧根组织发育的所有阶段都能高度检测到转录本。还证实，通过抑制IAA15蛋白的多聚泛素化，其对蛋白酶体介导的蛋白质降解具有很强的稳定性，从而导致生长素响应基因的转录抑制。特别是，作为侧根形成正调控因子的和的转录水平在IAA15 OX植物中显著受到抑制。此外，已阐明IAA15与ARF7和ARF19相互作用，并结合到和的启动子上，强烈表明IAA15通过抑制ARF7和ARF19的活性，对和进行转录抑制，从而抑制侧根形成。综上所述，本研究表明IAA15作为Aux/IAA-ARF模块的一个组成部分，在侧根形成中也起着关键的负向作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/83cb690cbc9e/fpls-11-01239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/61b7041df591/fpls-11-01239-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/b4128980a8ae/fpls-11-01239-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/6d6b9af821fb/fpls-11-01239-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/d4192b7c12fa/fpls-11-01239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/772e06e933b5/fpls-11-01239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/83cb690cbc9e/fpls-11-01239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/61b7041df591/fpls-11-01239-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/b4128980a8ae/fpls-11-01239-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/6d6b9af821fb/fpls-11-01239-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/d4192b7c12fa/fpls-11-01239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/772e06e933b5/fpls-11-01239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/7434933/83cb690cbc9e/fpls-11-01239-g006.jpg

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