Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou, 225009, People's Republic of China.
Shaanxi Origin Agricultural Science and Technology Co., Ltd, Tongchuan, 727000, People's Republic of China.
Planta. 2023 Jun 28;258(2):33. doi: 10.1007/s00425-023-04193-1.
The physiological and transcriptome analysis revealed that auxin was a positive regulator of lateral root development and tanshinone accumulation in Salvia miltiorrhiza. Roots of S. miltiorrhiza are widely used as medicinal materials in China, and the root morphology and content of bioactive compounds [such as phenolic acids and diterpenoid quinones (tanshinones)] are the main factors to determine the quality of this herb. Auxin regulates root development and secondary metabolism in many plant species, but little is known about its function in S. miltiorrhiza. In this study, S. miltiorrhiza seedlings were treated (exogenous application) with the auxin indole-3-acetic acid (IAA) and the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) to investigate the regulatory roles of auxin in S. miltiorrhiza. The results indicated that exogenous IAA promoted both lateral root development and tanshinones biosynthesis in S. miltiorrhiza. The NPA application suppressed the lateral root development but showed no obvious effects on tanshinones accumulation. Based on the RNA-seq analysis, expressions of genes related to auxin biosynthesis and signaling transduction were altered in both treated groups. Coincidental with the enhanced content of tanshinones, transcripts of several key enzyme genes in the tanshinones biosynthetic pathway were stimulated after the exogenous IAA application. The expression profiles of seven common transcription factor domain-containing gene families were analyzed, and the results implied that some AP2/ERF genes were probably responsible for the auxin-induced lateral root development in S. miltiorrhiza. These findings shed new light on the regulatory roles of auxin on root development and bioactive compounds biosynthesis in S. miltiorrhiza, and lay the groundwork for future research into the detailed molecular mechanism underlying these biological functions.
生理和转录组分析表明，生长素是丹参侧根发育和丹参酮积累的正向调节剂。丹参的根在中国被广泛用作药材，其根形态和生物活性化合物（如酚酸和二萜醌（丹参酮））的含量是决定这种草药质量的主要因素。生长素在许多植物物种中调节根的发育和次生代谢，但对其在丹参中的功能知之甚少。在这项研究中，用生长素吲哚-3-乙酸（IAA）和极性生长素运输抑制剂 1-萘基邻氨甲酰苯甲酸（NPA）处理丹参幼苗，以研究生长素在丹参中的调节作用。结果表明，外源性 IAA 促进了丹参侧根发育和丹参酮生物合成。NPA 的应用抑制了侧根发育，但对丹参酮积累没有明显影响。基于 RNA-seq 分析，生长素生物合成和信号转导相关基因的表达在这两个处理组中都发生了改变。与丹参酮含量的增加一致，外源性 IAA 处理后，丹参酮生物合成途径中的几个关键酶基因的转录本也被激活。对七个常见转录因子结构域基因家族的表达谱进行了分析，结果表明，一些 AP2/ERF 基因可能负责生长素诱导的丹参侧根发育。这些发现为生长素对丹参根发育和生物活性化合物生物合成的调节作用提供了新的认识，并为进一步研究这些生物学功能的详细分子机制奠定了基础。
