Kinoshita Ayaka, Koga Hiroyuki, Tsukaya Hirokazu
Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.
Front Plant Sci. 2020 Aug 12;11:1160. doi: 10.3389/fpls.2020.01160. eCollection 2020.
Members of the genus are unique in that they produce no new organ during the vegetative phase in the shoot; instead, one of the cotyledons grows indeterminately. The mechanism of this unique trait is unclear, in part because of the lack of suitable assessment techniques. We therefore established a whole-mount hybridization technique, a powerful means of examining spatial patterns in gene expression, for . By using this, we examined the expression pattern of a () ortholog, which is indispensable for the formation and maintenance of the shoot apical meristem (SAM) in typical angiosperms. Expression was confined to the groove meristem (GM), which corresponds to the SAM. We also assessed the expression pattern of (), a key promoter for cell division in the leaf meristem. It was expressed not only in the basal meristem (BM) tissue with active cell division in the basal part of the growing cotyledon but also in the GM. The findings suggest that the unusual gene expression pattern of the GM underpins the fuzzy morphogenesis of .
该属成员的独特之处在于，它们在茎的营养生长阶段不会产生新器官；相反，其中一片子叶会无限生长。这种独特性状的机制尚不清楚，部分原因是缺乏合适的评估技术。因此，我们为该属建立了一种整体杂交技术，这是一种检测基因表达空间模式的强大方法。通过使用这种技术，我们检测了一个直系同源基因的表达模式，该基因对于典型被子植物茎尖分生组织（SAM）的形成和维持是不可或缺的。表达局限于与SAM相对应的凹槽分生组织（GM）。我们还评估了叶片分生组织中细胞分裂关键启动子的表达模式。它不仅在生长子叶基部具有活跃细胞分裂的基部分生组织（BM）组织中表达，而且在GM中也有表达。这些发现表明，GM异常的基因表达模式是该属模糊形态发生的基础。
