Audu Kalen Ephraim, Adeniji Shola Elijah, Obidah John Solomon
Biology Department, Ahmadu Bello University, Zaria, Nigeria.
Chemistry Department, Ahmadu Bello University, Zaria, Nigeria.
Heliyon. 2020 Aug 14;6(8):e04704. doi: 10.1016/j.heliyon.2020.e04704. eCollection 2020 Aug.
Various clean-up techniques for heavy metals have been suggested and practiced for its biosorption from the contaminated or pollutant soil by using chemical and physical methods. But most of the methods are hazardous to the environment and expensive. This study was on how to determine the potential of resident bacteria in the removal of heavy metals from contaminated soils in Abare situated in Anka Local Government of Zamfara State, Nigeria. Thus, this study employed bioremediation technique for removal of heavy metals.
The preparation of Culture media and Isolation of bacteria of the different contaminated soils were achieved by spread plate method. Whereas, concentrations of the heavy metals (Lead (Pb), Copper (Cu) and Iron (Fe)) were determined by Atomic absorption spectrophotometer (AAS. was used for biosorption experiment. The concentrations of Pb ranged between 1.328 ± 0.493 to 2.326 ± 2.093 mg/L, Cu 0.234 ± 0.117 to 1.054 ± 1.486 mg/L and Fe 18.498 ± 11.462 to 27.754 ± 57.510 mg/L. The optimum temperature for biosorption condition was found to be 35 °C. More so, the optimum pH of (7) was observed for maximum biosorption of Pb and Cu ions by which may be attributed to homeostatic phenomenon and the availability of metal binding sites on the biosorbents. Metal uptake biosorption percentage revealed that absorbed 99.6% of Pb, 60% of Cu and 96% of Fe.
This study revealed that potential for bioremediation of the three metals.
针对从受污染或含有污染物的土壤中通过化学和物理方法进行生物吸附去除重金属，已提出并实践了各种清理技术。但大多数方法对环境有害且成本高昂。本研究旨在确定尼日利亚赞法拉州安卡地方政府阿巴雷地区受污染土壤中本地细菌去除重金属的潜力。因此，本研究采用生物修复技术去除重金属。
通过平板涂布法实现了不同污染土壤的培养基制备和细菌分离。而重金属（铅（Pb）、铜（Cu）和铁（Fe））的浓度通过原子吸收分光光度计（AAS）测定。 用于生物吸附实验。铅的浓度范围为1.328±0.493至2.326±2.093mg/L，铜为0.234±0.117至1.054±1.486mg/L，铁为18.498±11.462至27.754±57.510mg/L。发现生物吸附条件的最佳温度为35°C。此外，观察到最佳pH值为（7）时， 对铅和铜离子的生物吸附量最大，这可能归因于稳态现象以及生物吸附剂上金属结合位点的可用性。金属吸收生物吸附百分比显示， 吸附了99.6%的铅、60%的铜和96%的铁。
本研究揭示了 对这三种金属的生物修复潜力。
