Mogole Lebogang, Omwoyo Wesley, Mtunzi Fanyana
Department of Chemistry, Vaal University of Technology, Private Bag X021, Vandebijlpark, 1900, South Africa.
Department of Chemistry, Maasai Mara University, P.O Box 861-20500, Narok, Kenya.
Heliyon. 2020 Aug 21;6(8):e04736. doi: 10.1016/j.heliyon.2020.e04736. eCollection 2020 Aug.
Diabetes Mellitus is an endocrine disorder which causes insulin deficiency. Medicinal plants are documented to be efficacious in the management of the disease. The current research set to determine the phytochemicals present, anti-oxidant activity and investigate the potency of against α-amylase inhibition. The leaves of the plant were extracted sequentially. The different extracts were evaluated for the presence of phytochemicals and their potential anti-oxidant and α-amylase inhibition activity. Methanol with the highest polarity, gave the highest yield of 20% and hexane with the lowest polarity have the lowest yield of 2.09%. This trend resembled that observed for the total flavanoid and total phenolic content analysis which gave values of 0.3822 mg QAE/mg and 3.810 mg GAE/mg respectively for methanolic and hexane extracts. The extracts of methanol recorded higher DPPH free radical scavenging activity of 87% and gave the lowest IC of 0.5336 which was below that of ascorbic acid used as a control. Hexane extract had a higher α-amylase inhibitory activity of 24% at 1 μg/ml as compared to other extracts. Generally hexane extracts of exhibits mild inhibitory activity against α-amylase enzyme which is recommended than the conventional therapy which maximally inhibits the enzyme causing major side effects. The results obtained herein support the use of the plant as an anti-diabetic agent at higher concentrations.
糖尿病是一种导致胰岛素缺乏的内分泌紊乱疾病。有文献记载药用植物在该疾病的治疗中具有疗效。当前的研究旨在确定植物中存在的植物化学物质、抗氧化活性，并研究其对α -淀粉酶抑制的效力。对该植物的叶子进行了顺序提取。对不同提取物进行了植物化学物质的存在情况及其潜在抗氧化和α -淀粉酶抑制活性的评估。极性最高的甲醇提取物产率最高，为20%，极性最低的己烷提取物产率最低，为2.09%。这种趋势与总黄酮和总酚含量分析中观察到的相似，甲醇提取物和己烷提取物的总黄酮和总酚含量分别为0.3822毫克QAE/毫克和3.810毫克GAE/毫克。甲醇提取物的DPPH自由基清除活性更高，为87%，IC50最低，为0.5336，低于用作对照的抗坏血酸。与其他提取物相比，己烷提取物在1微克/毫升时对α -淀粉酶的抑制活性更高，为24%。总体而言，该植物的己烷提取物对α -淀粉酶表现出轻度抑制活性，与最大程度抑制该酶并导致主要副作用的传统疗法相比，更值得推荐。本文获得的结果支持在较高浓度下将该植物用作抗糖尿病药物。
