Shen Yuting, Chen Chuying, Cai Nan, Yang Ruopeng, Chen Jinyin, Kahramanoǧlu İbrahim, Okatan Volkan, Rengasamy Kannan R R, Wan Chunpeng
Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits, Vegetables/Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province, College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
College of Materials and Chemical Engineering, Pingxiang University, Pingxiang, China.
Front Nutr. 2021 Aug 20;8:663584. doi: 10.3389/fnut.2021.663584. eCollection 2021.
This study was performed to determine the antifungal activity of loquat ( Lindl) leaf extract (LLE) against the citrus postharvest pathogen (). The LLE exhibited an antifungal activity against , with a minimum inhibitory concentration (MIC) of 0.625 mg/ml and a minimum fungicidal concentration (MFC) of 1.25 mg/ml. Significant inhibitory effects of LLE on mycelial growth and spore germination of were seen in a dose-dependent manner. Simultaneously, to investigate possible antifungal mechanisms by LLE, we analyzed their influence on morphological changes, cell membrane permeability, cell wall and cell membrane integrity, and adenosine phosphates (ATP, ADP, and AMP) levels. Alterations, such as sunken surface and malformation, occurred in the LLE-treated spores. Furthermore, intracellular inclusion content decreased after LLE treatment, indicating an increase in cell membrane permeability. Besides, the LLE treatment induced a significant decline in the level of adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) with a noticeable addition of extracellular ATP, ADP, and AMP during the entire treatment period. Overall, the results manifested that the antifungal activity of LLE against can be attributed to the derangement of cell membrane permeability and disordered energy metabolism. This is the first report on the mechanism of antifungal activity of LLE and could be useful in the development of targeted fungicides from natural origin.
本研究旨在测定枇杷（Lindl）叶提取物（LLE）对柑橘采后病原菌（）的抗真菌活性。LLE对该病原菌表现出抗真菌活性，最低抑菌浓度（MIC）为0.625 mg/ml，最低杀菌浓度（MFC）为1.25 mg/ml。LLE对该病原菌菌丝生长和孢子萌发具有显著的剂量依赖性抑制作用。同时，为研究LLE可能的抗真菌机制，我们分析了其对形态变化、细胞膜通透性、细胞壁和细胞膜完整性以及腺苷磷酸（ATP、ADP和AMP）水平的影响。经LLE处理的该病原菌孢子出现了诸如表面凹陷和畸形等变化。此外，LLE处理后细胞内含物含量降低，表明细胞膜通透性增加。此外，LLE处理导致一磷酸腺苷（AMP）、二磷酸腺苷（ADP）和三磷酸腺苷（ATP）水平显著下降，且在整个处理期间细胞外ATP、ADP和AMP明显增加。总体而言，结果表明LLE对该病原菌的抗真菌活性可归因于细胞膜通透性紊乱和能量代谢失调。这是关于LLE抗真菌活性机制的首次报道，可能有助于开发天然来源的靶向杀菌剂。
