Saglie F R, Marfany A, Camargo P
School of Dentistry, University of California, Los Angeles, Los Angeles 90024.
J Periodontol. 1988 Apr;59(4):259-65. doi: 10.1902/jop.1988.59.4.259.
A total of six active and six nonactive sites from six untreated periodontitis patients were examined for intragingival presence of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis. The active destructive periodontal disease was determined by the "tolerance method." The method of immunoperoxidase was used in the identification of intragingival microorganisms in active and nonactive periodontal sites. Light microscopic sections of gingival tissues consecutive to those with gram stain, revealing presence of bacteria (substantiated by electron microscopy), were stained with peroxidase-labeled antibodies against A. actinomycetemcomitans and B. gingivalis. B. gingivalis was found to be significantly elevated in the connective tissue of active sites when compared to nonactive sites. A statistically significant border-line difference was found between active and nonactive sites in the connective tissue invaded by A. actinomycetemcomitans. Our findings plus the well established periodontopathic potential of A. actinomycetemcomitans and B. gingivalis support the concept that these bacteria are important invasive pathogenic agents in periodontitis.
对6名未经治疗的牙周炎患者的6个活动位点和6个非活动位点进行了检测，以确定牙龈内伴放线放线杆菌和牙龈拟杆菌的存在情况。活动性破坏性牙周病通过“耐受法”确定。免疫过氧化物酶法用于识别活动和非活动牙周位点中的牙龈内微生物。与革兰氏染色连续的牙龈组织光镜切片显示有细菌存在（经电子显微镜证实），用抗伴放线放线杆菌和牙龈拟杆菌的过氧化物酶标记抗体进行染色。与非活动位点相比，牙龈拟杆菌在活动位点的结缔组织中显著升高。在伴放线放线杆菌侵入的结缔组织中，活动位点和非活动位点之间存在统计学上显著的临界差异。我们的研究结果以及伴放线放线杆菌和牙龈拟杆菌已确定的牙周致病潜能支持了这样一种观点，即这些细菌是牙周炎中重要的侵入性致病因子。
