Saglie F R, Smith C T, Newman M G, Carranza F A, Pertuiset J H, Cheng L, Auil E, Nisengard R J
J Periodontol. 1986 Aug;57(8):492-500. doi: 10.1902/jop.1986.57.8.492.
Serial histological sections of gingiva obtained from each of six advanced adult periodontitis, two localized juvenile periodontitis and two periodontally healthy patients were used for specific identification of bacteria within the oral epithelium and adjacent connective tissue. Healthy gingival biopsies served as controls. Sections from patients and control biopsies were Gram-stained and also screened with antibacterial sera associated with the peroxidase immunocytochemical technique for specific bacterial identification. The "Pop-off" electron microscopic technique was also used to further demonstrate the bacterial nature of peroxidase-stained material. In addition, the possible correlation between bacteria and areas of possible reduced keratinization was investigated. The results showed that sections of orthokeratinized healthy gingiva did not contain bacteria. Gram-stained sections from diseased sites contained large numbers of bacteria in the oral epithelium and adjacent connective tissue. Bacteroides gingivalis and to a lesser extent Capnocytophaga gingivalis were found in periodontitis, and Actinobacillus actinomycetemcomitans was found in juvenile periodontitis when the immunoperoxidase technique was used. The bacterial nature of peroxidase-stained material was confirmed by the "pop-off" technique. In the disease biopsies, bacterial presence was correlated with areas of reduced amounts of keratin suggesting that the oral epithelium may be a portal of entry for bacteria into gingival tissues.
取自6例晚期成人牙周炎、2例局限性青少年牙周炎患者以及2例牙周健康患者的牙龈组织连续组织切片，用于特异性鉴定口腔上皮及相邻结缔组织中的细菌。健康牙龈活检组织作为对照。对患者切片和对照活检组织进行革兰氏染色，并采用与过氧化物酶免疫细胞化学技术相关的抗菌血清进行筛查，以进行特异性细菌鉴定。“弹出式”电子显微镜技术也用于进一步证实过氧化物酶染色物质的细菌性质。此外，还研究了细菌与可能角化减少区域之间的可能相关性。结果显示，正角化健康牙龈切片不含细菌。患病部位的革兰氏染色切片在口腔上皮和相邻结缔组织中含有大量细菌。使用免疫过氧化物酶技术时，在牙周炎中发现牙龈拟杆菌，在较小程度上发现牙龈二氧化碳嗜纤维菌，在青少年牙周炎中发现伴放线放线杆菌。过氧化物酶染色物质的细菌性质通过“弹出式”技术得以证实。在患病活检组织中，细菌的存在与角化量减少的区域相关，这表明口腔上皮可能是细菌进入牙龈组织的门户。
