Marendy Dominique, Baker Kim, Emery David, Rolls Peter, Stutchbury Ralph
Elanco Animal Health, Yarrandoo Research and Development Centre, New South Wales 2178, Australia.
Sydney School of Veterinary Science, University of Sydney, New South Wales 2006, Australia.
Vet Parasitol X. 2019 Dec 27;3:100022. doi: 10.1016/j.vpoa.2019.100022. eCollection 2020 May.
The intracellular protozoal parasite ikeda has rapidly spread across South-eastern Australia since 2006, causing deaths and production losses in cattle. The 3-host "bush tick" (Neumann) appears the principal biological vector in the endemic regions. To generate sufficient numbers of ticks to produce stabilate for infection to confirm vector competency and for acaricide trials, the optimal conditions and stage-specific intervals for the generational life-cycle of was defined on two dogs and two steers. To determine whether was a definitive host for nymphal stages were fed on a steer infected with and moulted adults were permitted to feed for 3 days on an uninfected calf prior to harvest. Subsequent detection of infection after inoculation of four naïve calves with stabilate produced from ground-up adult ticks or dissected salivary glands confirmed as one final (definitive) host for in Australia.
自2006年以来，细胞内原生动物寄生虫池田虫已在澳大利亚东南部迅速传播，导致牛死亡和生产损失。三宿主的“丛林蜱”（诺伊曼蜱）似乎是流行地区的主要生物传播媒介。为了繁殖足够数量的蜱以生产用于感染的冻干虫体制剂，从而确认传播媒介能力并进行杀螨剂试验，在两只狗和两头公牛身上确定了该蜱世代生命周期的最佳条件和特定阶段间隔。为了确定牛是否是若虫阶段的终末宿主，让若虫在感染了池田虫的公牛身上取食，在收获前，让蜕皮后的成虫在未感染的小牛身上取食3天。随后，用碾碎的成年蜱或解剖的唾液腺制成的冻干虫体制剂接种4只未感染的小牛后检测感染情况，证实牛是澳大利亚池田虫的终末（ definitive ）宿主之一。
